"
Team:KU Leuven/Project/StickerSystem
From 2013.igem.org
| (3 intermediate revisions not shown) | |||
| Line 39: | Line 39: | ||
<div class="span4 icon white"> | <div class="span4 icon white"> | ||
<div class="row-fluid"> | <div class="row-fluid"> | ||
| - | <div class="visible-desktop span3 bg- | + | <div class="visible-desktop span3 bg-green"> |
<a href="https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem"> | <a href="https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem"> | ||
<i class="livicon activeicon" data-name="refresh" data-color="white"></i> | <i class="livicon activeicon" data-name="refresh" data-color="white"></i> | ||
| Line 102: | Line 102: | ||
<div id="header" class="row-fluid"> | <div id="header" class="row-fluid"> | ||
<div class="span12"> | <div class="span12"> | ||
| - | <h3 class="bg- | + | <h3 class="bg-green">The Sticker System</h3> |
</div> | </div> | ||
</div> | </div> | ||
| Line 112: | Line 112: | ||
<div class="span12 white"> | <div class="span12 white"> | ||
<p align = "justify"> | <p align = "justify"> | ||
| - | Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our <b>BanAphids</b> could end up in the environment. <b>We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches</b>. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. <b>Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator.</b> The oscillator is extensively elaborated in the <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Modelling/Colony_Level | + | Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our <b>BanAphids</b> could end up in the environment. <b>We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches</b>. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. <b>Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator.</b> The oscillator is extensively elaborated in the <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Modelling/Colony_Level">oscillator model section</a></b>. Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.</p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 126: | Line 126: | ||
<div class="span10 icon-text "> | <div class="span10 icon-text "> | ||
| - | <h3> | + | <h3>Design</h3> </a> |
<div class="row-fluid"> | <div class="row-fluid"> | ||
<div class="span12"> | <div class="span12"> | ||
| Line 148: | Line 148: | ||
<div class="span10 icon-text "> | <div class="span10 icon-text "> | ||
| - | <h3> | + | <h3>Integrating the oscillator into the wetlab</h3> </a> |
<div class="row-fluid"> | <div class="row-fluid"> | ||
<div class="span12"> | <div class="span12"> | ||
Latest revision as of 03:19, 29 October 2013
Secret garden
Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!
- A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
- For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
- We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?
Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!
The Sticker System
Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our BanAphids could end up in the environment. We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator. The oscillator is extensively elaborated in the oscillator model section. Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.
