Team:EPF Lausanne/Calendar/28 October 2013

From 2013.igem.org

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Repeating the microscopy
Repeating the microscopy
<br>- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
<br>- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
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<br>- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-GFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-GFP antibody (revealed with avidin dylight (red)).
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<br>- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-YFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-YFP antibody (revealed with avidin dylight (red)).
<br>- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.
<br>- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.
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<font size = "4"> Nanoparticles</font> <BR>
<font size = "4"> Nanoparticles</font> <BR>

Latest revision as of 23:43, 28 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display


CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)

Repeating the microscopy
- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-YFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-YFP antibody (revealed with avidin dylight (red)).
- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.




Nanoparticles

Microfluidic
- Repeat microfluidics experiment for nanoparticles digestion with MMP2

Wiki Links

Wiki Links

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