Team:Wageningen UR

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<!--- Header Template --->
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{{:Team:Wageningen_UR/Templates/Page Header}}
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<!--- Navigation Template --->
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{{:Team:Wageningen_UR/Templates/Navigation}}
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<div class="slider">
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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        <ul id="example3" class="timeliner">
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This is a template page. READ THESE INSTRUCTIONS.
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<li title="Aspergillus Nigem" lang="5">
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<span>Bricks - Devices - Host</span>
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                        <div><a href="https://2013.igem.org/Team:Wageningen_UR/Why_Aspergillus_nigem">Learn more</a></div>
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</li>
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<li title="Secondary metabolites" lang="5">
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<span>A modular approach</span>
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                        <div><a href="https://2013.igem.org/Team:Wageningen_UR/Secondary_metabolites">Learn more</a></div>
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</li>
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<li title="Toolbox" lang="5">
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<span><i>In vivo</i> diagnostics</span>
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                        <div><a href="https://2013.igem.org/Team:Wageningen_UR/Chromoproteins">Learn more</a></div>
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</li>
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<li title="Host engineering" lang="5">
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<span>Generating single cell factories</span>
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                        <div><a href="https://2013.igem.org/Team:Wageningen_UR/Engineering_morphology">Learn more</a></div>
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</li>
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<li title="Human Practices" lang="5">
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<span>Science is rooted in Society<br/>Harvest the opportunities before they hit you</span>
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                        <div><a href="https://2013.igem.org/Team:Wageningen_UR/Vitruvian_man">Learn more</a></div>
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                </li>
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<li title="Team">
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<span>Team</span>
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                        <div><a href="https://2013.igem.org/Team:Wageningen_UR/Team">Meet them</a></div>
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</li>
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</ul>
</div>
</div>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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<div class="projecttour">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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    <a href="https://2013.igem.org/Team:Wageningen_UR/Why_Aspergillus_nigem" class="tour"><span class="startt">Start</span><span class="tourr">Tour</span></a>
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.
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<div class="twt">
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    <a href="https://twitter.com/iGemWUR2013" class="twitter-follow-button" data-show-count="false">Follow @iGemWUR2013</a>
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</div>
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</html>
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<div id="wrapper">
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<div class="half left">
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<hr/></html>
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<!-- *** End of the alert box *** -->
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==Introduction==
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<html>
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<p class="intro">The discovery of secondary metabolites has had a profound impact on the development of human medicines such as penicillins, cephalosporins. However, the widespread deployment of pharmaceuticals derived from secondary metabolites is severely limited by our ability to implement economically viable large-scale production systems. While advances in metabolic engineering and process technology have allowed the biobased production of fuels and chemicals to occur at an unprecedented scale, the production of many useful secondary metabolites has lagged behind. This is in part due to the slow growth and low productivity of the organisms that naturally produce these compounds, but also due to the complexity of the biochemical pathways involved in their biosynthesis. Therefore, using synthetic biology tools to identify, optimize and transplant these biosynthetic pathways into more productive host organisms is a major opportunity to overcome current technological limitations and ultimately to disseminate crucial medical treatments to people without the means to afford pharmaceuticals produced via traditional organic synthesis. <br/><br/>
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In order to pave the way for next-generation metabolic engineering, it was necessary to go beyond established standards. <i>Escherichia coli</i> is undoubtedly the most well-characterized organism in existence and has been the primary workhorse of fundamental synthetic biology research. However, despite the spectacular achievements in understanding and manipulating all levels of its physiology, when it comes to industrial-scale production, <i>E. coli</i> is far from an ideal cell factory. In contrast, yeasts and fungi are naturally more robust, more productive, and are easier to separate in downstream processing. The filamentous fungus <i>Aspergillus niger</i> is widely used in the production of organic acids due to its unmatched ability to secrete metabolites. Due to its inherent biosynthetic capabilities, the recent development of effective genome engineering tools makes it an ideal platform for advanced metabolic engineering applications. <br/><br/>
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In order to establish <i>A. niger</i> as a viable platform organism, we took a threefold approach:<br/>
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a) The modularization and refactoring of a heterologous biosynthetic enzyme complex. <br/>
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b) The development of a versatile molecular toolkit to facilitate the analysis of its physiology including in-situ ATP and pH biosensors, chromoproteins to screen transformants, and fluorescent cytoskeleton proteins to visualize <i>A. niger</i>’s cellular structure.<br/>
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c) A r-evolutionary engineering method to develop a host strain with reduced mycelial cohesiveness for improved productivity.</p>
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<hr/></html>
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==Achievements==
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<html>
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<div class="achievements">
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    <div class="achieve bronze">
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Achievements"><img src="https://static.igem.org/mediawiki/2013/f/f2/Bronze.png" alt="Bronze medal">Bronze medal</a>
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        <div class="moreinfo">
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            <p class="check">Team registration</p>
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            <p class="check">Complete Judging form</p>
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            <p class="check">Team Wiki</p>
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            <p class="check">Present a poster and a talk at the iGEM Jamboree</p>
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            <p class="check">Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry</p>
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        </div>
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    </div>
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    <div class="achieve silver">
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Achievements"><img src="https://static.igem.org/mediawiki/2013/0/0b/Silver.png" alt="Silver medal">Silver medal</a>
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        <div class="moreinfo">
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            <p class="check">Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected</p>
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            <p class="check">Submit this new part to the iGEM Parts Registry</p>
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            <p class="check">Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project</p>
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        </div>
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    </div>
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    <div class="achieve gold">
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Achievements"><img src="https://static.igem.org/mediawiki/2013/3/32/Goldmed.png" alt="Silver medal">Gold medal</a>
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        <div class="moreinfo">
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            <p class="check">Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system</p>
 +
            <p class="check">Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe a novel approach that your team has used to help you and others consider these aspects of the design and outcomes of synthetic biology efforts. Please justify its novelty and how this approach might be adapted and scaled for others to use</p>
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            <p class="check">Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).</p>
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{|align="justify"
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        </div>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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    </div>
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|[[Image:Wageningen_UR_logo.png|200px|right|frame]]
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    <div class="clearfix"></div>
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</div>
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</div>
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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<div class="half right">
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|[[Image:Wageningen_UR_team.png|right|frame|Your team picture]]
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<hr/></html>
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|-
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|align="center"|[[Team:Wageningen_UR | Team Wageningen_UR]]
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<!--- The Mission, Experiments --->
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==Project navigation==
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<html><div class="outline">
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        <div class="cell one">
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Secondary_metabolites"><img src="https://static.igem.org/mediawiki/2013/4/41/Secmet.png" alt="Secondary metabolites"/></a></span>
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Lovastatin"><img src="https://static.igem.org/mediawiki/2013/4/42/Lov.png" alt="Lovastatin"/></a></span>
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Modeling"><img src="https://static.igem.org/mediawiki/2013/b/b2/Mtlab.png" alt="Modeling"/></a></span>
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Chromoproteins"><img src="https://static.igem.org/mediawiki/2013/b/bc/Chro.png" alt="Chromoproteins"/></a></span>
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Infrastructure"><img src="https://static.igem.org/mediawiki/2013/d/d9/Cyt.png" alt="Infrastructure"/></a></span>
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Biosensors"><img src="https://static.igem.org/mediawiki/2013/3/3a/Biosens.png" alt="Biosensors"/></a></span>
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            <span><a href="https://2013.igem.org/Team:Wageningen_UR/Host_engineering"><img src="https://static.igem.org/mediawiki/2013/b/b5/Hostcell.png" alt="Host engineering"/></a></span>
 +
        </div>
 +
        <div class="text one">
 +
            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Secondary_metabolites">Secondary metabolites</a></span>
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            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Lovastatin">Lovastatin</a></span>
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            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Modeling">Modeling</a></span>
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            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Chromoproteins">Chromoproteins</a></span>
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            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Infrastructure">Infrastructure</a></span>
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            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Biosensors">Biosensors</a></span>
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            <span class="line"><a href="https://2013.igem.org/Team:Wageningen_UR/Host_engineering">Host engineering</a></span>
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        </div>
 +
    </div>
 +
    <div class="scale">
 +
            <a href="#" id="one" class="on">Secondary metabolites</a>
 +
            <a href="#" id="two">Toolbox</a>
 +
            <a href="#" id="three">Host engineering</a>
 +
            <a href="" target="_blank" id="videolink">Project video</a>
 +
            <div class="clearfix"></div>
 +
    </div>
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<hr/></html>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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==Scientific Interactions==
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!align="center"|[[Team:Wageningen_UR|Home]]
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<html>
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!align="center"|[[Team:Wageningen_UR/Team|Team]]
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    <div class="vitruvian">
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Wageningen_UR Official Team Profile]
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Vitruvian_man"><img src="https://static.igem.org/mediawiki/2013/6/6b/Vitruvian.png" alt="vitruvian man">Vitruvian man</a>
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!align="center"|[[Team:Wageningen_UR/Project|Project]]
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        <a href="https://2013.igem.org/Team:Wageningen_UR/IgemNL"><img src="https://static.igem.org/mediawiki/2013/4/40/Igemnl.png" alt="iGEM Netherlands">iGEM Netherlands</a>
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!align="center"|[[Team:Wageningen_UR/Parts|Parts Submitted to the Registry]]
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Conference"><img src="https://static.igem.org/mediawiki/2013/8/87/Posterpres.png" alt="Poster presentation">Poster presentation</a>
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!align="center"|[[Team:Wageningen_UR/Modeling|Modeling]]
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Masterclass"><img src="https://static.igem.org/mediawiki/2013/7/7b/Discpsyc.png" alt="Masterclass">Masterclass</a>
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!align="center"|[[Team:Wageningen_UR/Notebook|Notebook]]
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Collaborations"><img src="https://static.igem.org/mediawiki/2013/1/12/Collabs.png" alt="Collaborations">Collaborations</a>
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!align="center"|[[Team:Wageningen_UR/Safety|Safety]]
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        <a href="https://2013.igem.org/Team:Wageningen_UR/Science_cafe"><img src="https://static.igem.org/mediawiki/2013/f/f9/Sciencecafe.png" alt="Science café">Science café</a>
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!align="center"|[[Team:Wageningen_UR/Attributions|Attributions]]
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    </div>
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|}
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</div>
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            <div class="clearfix"></div>
 +
        </div>
 +
</div>
 +
<div id="sponsors">
 +
        <h2>Sponsors</h2>
 +
        <a href="https://www.facebook.com/beauvillemedia" target="_blank" class="sponsor" title="Beauville Media"><img src="https://static.igem.org/mediawiki/2013/5/5e/Beauville-media.png" alt="Beauville Media" style="display:block; vertical-align:bottom;"/></a>
 +
        <a href="http://www.wageningenur.nl/" target="_blank" class="sponsor" title="Wageningen UR"><img src="https://static.igem.org/mediawiki/2013/1/10/Wageningen_ur.png" alt="Wageningen UR" style="display:block; vertical-align:bottom;"/></a>
 +
        <a href="https://www.bioke.com/" target="_blank" class="sponsor" title="Bioké"><img src="https://static.igem.org/mediawiki/2013/c/c3/Bioke.png" alt="Bioké" style="display:block; vertical-align:bottom;"/></a>
 +
        <a href="http://www.geneious.com/" target="_blank" class="sponsor" title="Genious"><img src="https://static.igem.org/mediawiki/2013/d/db/Geneious.png" alt="Geneious" style="display:block; vertical-align:bottom;"/></a>
 +
        <a href="http://www.olympus.nl/" target="_blank" class="sponsor" title="Olympus"><img src="https://static.igem.org/mediawiki/2013/4/4d/Olympus.png" alt="Olympus" style="display:block; vertical-align:bottom;"/></a>
 +
        <a href="https://www.neb.com/" target="_blank" class="sponsor" title="New England Biolabs inc."><img src="https://static.igem.org/mediawiki/2013/a/ad/Biolabs.png" alt="New England Biolabs inc." style="display:block; vertical-align:bottom;"/></a>
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        <a href="http://www.dsm.com/" target="_blank" class="sponsor" title="DSM"><img src="https://static.igem.org/mediawiki/2013/4/4a/Dsm.png" alt="DSM" style="display:block; vertical-align:bottom;"/></a>
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        <a href="http://www.vlaggraduateschool.nl/" target="_blank" class="sponsor" title="Graduate School VLAG"><img src="https://static.igem.org/mediawiki/2013/0/02/Vlag.png" alt="Graduate School VLAG" style="display:block; vertical-align:bottom;"/></a>
 +
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Latest revision as of 03:50, 5 October 2013

StartTour

Introduction

The discovery of secondary metabolites has had a profound impact on the development of human medicines such as penicillins, cephalosporins. However, the widespread deployment of pharmaceuticals derived from secondary metabolites is severely limited by our ability to implement economically viable large-scale production systems. While advances in metabolic engineering and process technology have allowed the biobased production of fuels and chemicals to occur at an unprecedented scale, the production of many useful secondary metabolites has lagged behind. This is in part due to the slow growth and low productivity of the organisms that naturally produce these compounds, but also due to the complexity of the biochemical pathways involved in their biosynthesis. Therefore, using synthetic biology tools to identify, optimize and transplant these biosynthetic pathways into more productive host organisms is a major opportunity to overcome current technological limitations and ultimately to disseminate crucial medical treatments to people without the means to afford pharmaceuticals produced via traditional organic synthesis.

In order to pave the way for next-generation metabolic engineering, it was necessary to go beyond established standards. Escherichia coli is undoubtedly the most well-characterized organism in existence and has been the primary workhorse of fundamental synthetic biology research. However, despite the spectacular achievements in understanding and manipulating all levels of its physiology, when it comes to industrial-scale production, E. coli is far from an ideal cell factory. In contrast, yeasts and fungi are naturally more robust, more productive, and are easier to separate in downstream processing. The filamentous fungus Aspergillus niger is widely used in the production of organic acids due to its unmatched ability to secrete metabolites. Due to its inherent biosynthetic capabilities, the recent development of effective genome engineering tools makes it an ideal platform for advanced metabolic engineering applications.

In order to establish A. niger as a viable platform organism, we took a threefold approach:
a) The modularization and refactoring of a heterologous biosynthetic enzyme complex.
b) The development of a versatile molecular toolkit to facilitate the analysis of its physiology including in-situ ATP and pH biosensors, chromoproteins to screen transformants, and fluorescent cytoskeleton proteins to visualize A. niger’s cellular structure.
c) A r-evolutionary engineering method to develop a host strain with reduced mycelial cohesiveness for improved productivity.


Achievements

Bronze medalBronze medal

Team registration

Complete Judging form

Team Wiki

Present a poster and a talk at the iGEM Jamboree

Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry

Silver medalSilver medal

Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected

Submit this new part to the iGEM Parts Registry

Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project

Silver medalGold medal

Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system

Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe a novel approach that your team has used to help you and others consider these aspects of the design and outcomes of synthetic biology efforts. Please justify its novelty and how this approach might be adapted and scaled for others to use

Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).

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