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Team:Paris Bettencourt/Notebook/Phage Sensor/Thursday 1st August.html
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| + | <TABLE BORDER> | ||
| + | <TR><TD><b>Reagent</b></TD><TD><b>Volume</b></TD></TR> | ||
| + | <TR><TD> </TD><TD><b>1x</b></TD></TR> | ||
| + | <TR><TD>Nuclease-free water</TD><TD>37.25 µL</TD></TR> | ||
| + | <TR><TD>5x Phusion HF Buffer</TD><TD>10 µL</TD></TR> | ||
| + | <TR><TD>10 mM dNTPs</TD><TD>1 µL</TD></TR> | ||
| + | <TR><TD>Forward Primer (10 µM)</TD><TD>0.5 µL</TD></TR> | ||
| + | <TR><TD>Reverse Primer (10 µM)</TD><TD>0.5 µL</TD></TR> | ||
| + | <TR><TD>Template Plasmid</TD><TD>0.25 µL</TD></TR> | ||
| + | <TR><TD>Phusion DNA Polymerase</TD><TD>0.5 µL</TD></TR> | ||
| + | <TR><TD><b>Total Volume</b></TD><TD>50 µL</TD></TR> | ||
| + | </TABLE> | ||
| + | <br><br> | ||
Gradient:<br> | Gradient:<br> | ||
Pos 1: 40°<br> | Pos 1: 40°<br> | ||
Latest revision as of 10:13, 23 August 2013
Phage Sensor
ASDFThursday 1st August
PCR (gradient + usual) of SPCR1, SPCR7, SPCR8, SPCR10, SPCR9, SPCR4
| Reagent | Volume |
| 1x | |
| Nuclease-free water | 37.25 µL |
| 5x Phusion HF Buffer | 10 µL |
| 10 mM dNTPs | 1 µL |
| Forward Primer (10 µM) | 0.5 µL |
| Reverse Primer (10 µM) | 0.5 µL |
| Template Plasmid | 0.25 µL |
| Phusion DNA Polymerase | 0.5 µL |
| Total Volume | 50 µL |
Gradient:
Pos 1: 40°
Pos 2: 40,2°
Pos 3: 41,3°
Pos 4: 43,1°
Pos 5: 45,4°
Pos 6: 48°
Pos 7: 50,7°
Pos 8: 53,5°
Pos 9: 56,0°
Pos 10: 58,1°
Pos 11: 59,8°C
(Pos 12: 60,6°)
Usual PCR: 52°
