06/08/13

From 2013.igem.org

(Difference between revisions)
(Created page with "==Running of an agarose gel== *Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.")
 
(5 intermediate revisions not shown)
Line 1: Line 1:
 +
<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
 +
{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
 +
!align="center"|[[Team:Leicester|Home]]
 +
!align="center"|[[Team:Leicester/Team|Team]]
 +
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
 +
!align="center"|[[Team:Leicester/Project|Project]]
 +
!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
 +
!align="center"|[[Team:Leicester/Modeling|Modeling]]
 +
!align="center"|[[Team:Leicester/Notebook|Notebook]]
 +
!align="center"|[[Team:Leicester/Safety|Safety]]
 +
!align="center"|[[Team:Leicester/Attributions|Attributions]]
 +
|}
 +
</div>
 +
 +
<p>
==Running of an agarose gel==
==Running of an agarose gel==
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
 +
*expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)
 +
[[File:igem_single_dig_060813.jpg]]
 +
*The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected.
 +
*No bands appeared for samples 5.1.
 +
*For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized.
 +
 +
==Making chloroamphenicol agar plates==
 +
*Making additional plates
 +
**400ml of agar
 +
***Melt in microwave
 +
***3mins on low -> shake
 +
***Repeat
 +
***2mins on low -> shake
 +
***Repeat
 +
**Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
 +
**Pour out onto 20 petri dishes
 +
**Leave to set on bench
 +
 +
==Plating out samples==
 +
*Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates.
 +
*Grow overnight at 37C

Latest revision as of 14:31, 6 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Running of an agarose gel

  • Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
  • expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)

Igem single dig 060813.jpg

  • The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected.
  • No bands appeared for samples 5.1.
  • For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized.

Making chloroamphenicol agar plates

  • Making additional plates
    • 400ml of agar
      • Melt in microwave
      • 3mins on low -> shake
      • Repeat
      • 2mins on low -> shake
      • Repeat
    • Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
    • Pour out onto 20 petri dishes
    • Leave to set on bench

Plating out samples

  • Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates.
  • Grow overnight at 37C

Retrieved from "http://2013.igem.org/06/08/13"