Team:AITM-Nepal

From 2013.igem.org

(Difference between revisions)
 
(190 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
-
 
<html>
<html>
-
<div id="box" style="width: 823px; height:277px margin-left: 137px; padding-top: 25px; padding-left: 120px; align:center; background-color:#000;">
+
<head>
 +
<style>
-
<img src="https://static.igem.org/mediawiki/2013/c/cd/AITM-Nepal_team.png"/>
+
/* Igm Css  Override */
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
#catlinks,#top-section,#footer-box,#top,#p-logo
 +
{
 +
display:none;
 +
}
 +
 
 +
h1
 +
{
 +
display:none;
 +
}
 +
 +
/* Css Override Complete */
 +
 
 +
/* body layout start */
 +
 
 +
body
 +
{
 +
background:#aaaaaa;
 +
}
 +
 
 +
 
 +
#content
 +
{
 +
background:#aaaaaa;
 +
width:auto;
 +
}
 +
 
 +
.AllContent
 +
{
 +
background:#FFFFFF;
 +
margin:auto;
 +
width:1050px;
 +
-webkit-box-shadow:rgb(110,110,110) 8px 8px 8px;
 +
}
 +
 +
        .maincontents
 +
        {
 +
        padding:10px;
 +
        }
 +
 
 +
/* Body layout Complete */
 +
 
 +
/* Logos css start */
 +
 
 +
      .header
 +
        {
 +
      padding:20px;
 +
        }
 +
 
 +
#logo
 +
{
 +
width:150px;
 +
height:150px;
 +
margin-right:100px;
 +
}
 +
 +
#logo2
 +
{
 +
float:right;
 +
width:200px;
 +
height:150px;
 +
}
 +
 
 +
/* Logos css Complete */
 +
 
 +
/* Navbar css start */
 +
 
 +
 
 +
.navbar
 +
{
 +
background:-webkit-linear-gradient(Top,#666,#000);
 +
width:1048px;
 +
height:50px;
 +
border:#000000 1px solid;
 +
-webkit-box-shadow:rgb(110,110,110) 0px 8px 8px;
 +
margin-bottom:20px;
 +
}
 +
 
 +
#navigationboxholder
 +
{
 +
        margin:auto;
 +
        width:1026px;
 +
        height:48px;
 +
        padding:10px;
 +
}
 +
 
 +
#navigation li
 +
{
 +
display:inline;
 +
list-style:none;
 +
}
 +
 
 +
ul li .navbar-option
 +
{
 +
color:#FFF;
 +
padding-left:10px;
 +
padding-right:10px;
 +
padding-bottom:10px;
 +
padding-top:10px;
 +
text-decoration:none;
 +
}
 +
 
 +
ul li .navbar-option:hover
 +
{
 +
background:#0FF;
 +
border-top:black 1px solid;
 +
border-left:black 1px solid;
 +
padding-left:9px;
 +
padding-top:9px;
 +
color:#000;
 +
text-decoration:underline;
 +
-webkit-transition: background 1s, color 1s;
 +
}
 +
 
 +
.sub_navigation
 +
{
 +
display:none;
 +
list-style-type:none;
 +
padding:10px;
 +
position:absolute;
 +
background:#000000;
 +
color:#FFF;
 +
-webkit-box-shadow:rgb(110,110,110) 0px 8px 8px;
 +
}
 +
 
 +
ul.sub_navigation li
 +
{
 +
clear:both;
 +
}
 +
 
 +
 
 +
 
 +
/* navbar css complete */
 +
 
 +
/*Image Slider css start */
 +
 
 +
#box
 +
{
 +
overflow:hidden;
 +
text-align:center;  
 +
background-color:#2d2d2d;
 +
height:400px;
 +
width:900px;
 +
margin:auto;
 +
border-bottom:30px #666666 groove;
 +
border-bottom-left-radius:20px;
 +
border-bottom-right-radius:20px;
 +
border-top:30px #666666 groove;
 +
border-top-left-radius:20px;
 +
border-top-right-radius:20px;
 +
margin-top:20px;
 +
}
 +
 
 +
/* Next and Previous Button css start */
 +
 +
#smallbox1
 +
{
 +
position:absolute;
 +
margin-top:180px;
 +
float:left;  
 +
padding:20px;  
 +
background:black;
 +
opacity:0.5;
 +
}
 +
 +
#smallbox2
 +
{
 +
position:absolute;
 +
margin-top:180px;
 +
margin-left:850px;
 +
padding:20px;
 +
background:black;
 +
opacity:0.5;
 +
}
 +
 +
#smallbox1:hover
 +
{
 +
opacity:0.8;
 +
}
 +
 +
#smallbox2:hover
 +
{
 +
opacity:0.8;
 +
}
 +
 +
/* Next and Previous Button css Complete */
 +
 +
/*Image Slider css Complete */
 +
 
 +
</style>
 +
 
 +
<!-- Image Slider JavaScript Start -->
 +
 
 +
<script type="text/javascript">
 +
   
 +
var number=0;
 +
 +
var img = new Array(2);
 +
img[0] = new Image();
 +
img[0].src = "https://static.igem.org/mediawiki/2013/c/c0/Aitmbanner_%282%29.png";
 +
 +
img[1] = new Image();
 +
img[1].src= "https://static.igem.org/mediawiki/2013/c/c0/Aitmbanner_%282%29.png";
 +
 +
img[2] = new Image();
 +
img[2].src= "https://static.igem.org/mediawiki/2013/c/c0/Aitmbanner_%282%29.png";
 +
   
 +
   
 +
   
 +
    function next(){
 +
number ++;
 +
if (number>0){
 +
number=0;
 +
}
 +
document.slider.src = img[number].src;
 +
        }
 +
       
 +
    function prev(){
 +
number --;
 +
if (number <0){
 +
number = 2;
 +
}
 +
document.slider.src= img[number].src;
 +
}
 +
   
 +
    var intervalId = setInterval(function(){
 +
number --;
 +
if (number <0){
 +
number = 2;
 +
}
 +
document.slider.src= img[number].src;
 +
        }, 4000);
 +
 
 +
 
 +
 +
    </script>
 +
 
 +
<!-- Image Slider JavaScript Complete -->
 +
 
 +
<script>
 +
 
 +
function togglemenu()
 +
{
 +
$('.sub_navigation').slideToggle();
 +
}
 +
 
 +
</script>
 +
 
 +
 
 +
 
 +
</head>
 +
 
 +
<body>
 +
 
 +
<div class="AllContent">
 +
 
 +
<!-- Jquery src start -->
 +
 
 +
<script src="http://code.jquery.com/jquery-1.10.2.min.js"></script>
 +
<script src="http://code.jquery.com/jquery-1.10.2.js"></script>
 +
 
 +
<!-- Jquery src Complete -->
 +
 
 +
<!-- Logo Section Start -->
 +
 
 +
<div class="header">             
 +
    <img id="logo" src="https://static.igem.org/mediawiki/2013/d/d1/Hava1.png">
 +
<img src="https://static.igem.org/mediawiki/2013/8/8e/1186269_700314813316746_1614415077_n.png" width="500px" height="140px" style="margi:auto;">
 +
    <a href="https://2013.igem.org/Main_Page"><img id="logo2" src="https://static.igem.org/mediawiki/2013/e/e3/Wiki1.png"></a>
</div>
</div>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
 
 +
<!-- Logo Section Complete -->
 +
 
 +
<!-- Navbar Section Start -->
 +
 
 +
<div class="navbar">
 +
    <div id="navigationboxholder">
 +
        <ul id="navigation">
 +
               
 +
                <li><a  class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal">Home</a></li>
 +
                <li><a  class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Team">Team</a></li>
 +
                <li><a onMouseOver="togglemenu();" href="https://2013.igem.org/Team:AITM-Nepal/Project" class="navbar-option">Project</a>
 +
                            <ul class="sub_navigation">
 +
                                    <li><a href="https://2013.igem.org/Team:AITM-Nepal/Overview" class="navbar-option">Overview</a></li>
 +
                                    <li><a href="https://2013.igem.org/Team:AITM-Nepal/Device_made" class="navbar-option">Device Made</a></li>
 +
                                    <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part1" class="navbar-option">Part 1</a></li>
 +
                                    <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part2" class="navbar-option">Part 2</a></li>
 +
                                    <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part3" class="navbar-option">Part 3</a></li>
 +
                            </ul></li>
 +
                <li><a class="navbar-option" href="https://igem.org/Team.cgi?year=2013&team_name=AITM-Nepal">Official Team Profile</a></li>
 +
                <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Parts">Parts Submitted To Registry</a></li>
 +
                <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Notebook">Notebook</a></li>
 +
                <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Safety">Safety</a></li>
 +
                <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Human_practise">Human Practice</a></li>
 +
                <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Attributions">Attributions</a></li>
 +
 +
    </ul>
 +
  </div>
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
</div>
 
-
</div>
 
-
</html>
 
-
<!-- *** End of the alert box *** -->
+
<!-- Navbar Section Complete -->
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
-
!align="center"|[[Team:AITM-Nepal|Home]]
+
-
!align="center"|[[Team:AITM-Nepal/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=AITM-Nepal Official Team Profile]
+
-
!align="center"|[[Team:AITM-Nepal/Project|Project]]
+
-
!align="center"|[[Team:AITM-Nepal/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:AITM-Nepal/Modeling|Modeling]]
+
-
!align="center"|[[Team:AITM-Nepal/Notebook|Notebook]]
+
-
!align="center"|[[Team:AITM-Nepal/Safety|Safety]]
+
-
!align="center"|[[Team:AITM-Nepal/Attributions|Attributions]]
+
-
|}
+
-
<div style="padding-top:25px;"></div>
 
-
<h3>immunEcoli</h3>
 
-
This is the first approach for Synthetic Biology from Nepal. We are a team of Undergrads of Biotechnology from Asian Institute of Technology and Management. This year we are trying to engineer Escherichia coli which will be used as a carrier and immune modulation point using Outer Membrane Vesicle (OMVs).Escherichia coli, being a model organism, is easier to work with , especially for the purpose of genetically engineered realm.
+
                   
 +
 
 +
<!-- Igm Javascript Start -->
 +
 
 +
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
 +
   
 +
   
 +
   
 +
            <div id="menubar" class="left-menu noprint">
 +
              <ul>
 +
                              <li class="selected"><a href="/Team:AITM-Nepal">Page              </a></li>
 +
                      <li class="new"><a href="/wiki/index.php?title=Talk:Team:AITM-Nepal&amp;action=edit&amp;redlink=1">Discussion              </a></li>
 +
                      <li><a href="/wiki/index.php?title=Team:AITM-Nepal&amp;action=edit">Edit              </a></li>
 +
                      <li><a href="/wiki/index.php?title=Team:AITM-Nepal&amp;action=history">History              </a></li>
 +
                      <li><a href="/Special:MovePage/Team:AITM-Nepal">Move              </a></li>
 +
                      <li><a href="/wiki/index.php?title=Team:AITM-Nepal&amp;action=unwatch">Unwatch              </a></li>
 +
                      <li style="color:white;cursor:default">teams</li>
 +
              </ul>
 +
            </div> <!-- end menubar (left) -->
 +
   
 +
            <div class="right-menu noprint" id="menubar">
 +
                <ul>
 +
                                        <li id="pt-userpage"><a href="/User:Gmanandhar" title="Your user page [.]" accesskey="." class="new">Gmanandhar</a></li>                              <li id="pt-preferences"><a href="https://igem.org/User_Information" title="My account">My account</a></li>
 +
                                                <li id="pt-logout"><a href="/wiki/index.php?title=Special:UserLogout&amp;returnto=Team:AITM-Nepal" title="Log out">Log out</a></li>     </ul>
 +
            </div><!-- end right menubar -->
 +
    <!--
 +
        <div id="search-controls" class="noprint">
 +
            <form action="/Special:Search" id="searchform">
 +
            <input id="searchInput" name="search" type="text" title="Search 2013.igem.org [alt-f]" accesskey="f" value="" autocomplete="off">
 +
                <input type="submit" name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists">&nbsp;
 +
                  <input type="submit" name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text">
 +
            </form>
 +
        </div> <!-- close search controls -->
 +
 
 +
<!-- Igm Javascript Ends -->                           
 +
 
 +
<div class="maincontents">
 +
 
 +
<!-- Image Slider Section Starts -->
 +
 
 +
    <div id="box">
 +
        <div id="smallbox1"><font color="white"><a href="" onClick="prev();"><</a></font></div>
 +
        <div id="smallbox2"><font color="white"><a href="" onClick="next();">></a></font></div>
 +
        <img src="https://static.igem.org/mediawiki/2013/c/c0/Aitmbanner_%282%29.png" width=100% height=400px name="slider"/>
 +
    </div>
 +
   
 +
<!-- Image Slider Section Complete -->
 +
 
 +
<br/>
 +
 
 +
<!-- Main Content Section Starts -->
 +
 
 +
<div>
 +
 
 +
    <a href="http://www.aitm.edu.np"><img id="logo3" src="https://static.igem.org/mediawiki/2013/2/27/Team_AITM-NEPAL.gif"></a><br><br>
 +
   
 +
    <h2><b><font color="#000000" face="Georgia">Our Project:</font></b></h2>
 +
   
 +
    <h3><b><font color="#000000" face="Georgia">ImmunEcoli</b></h3>
 +
   
 +
    This is the first approach for Synthetic Biology from Nepal. We are a team of Undergrads of Biotechnology from Asian Institute of Technology and Management. This year we are trying to engineer ''Escherichia coli'' which will be used as a carrier and immune modulation point using Outer Membrane Vesicle (OMVs). ''Escherichia coli'', being a model organism, is easier to work with , especially for the purpose of genetically engineered realm.
 +
   
 +
    Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. The delivery of siRNA in a packaged outer membrane vesicle of gram negative bacteria is the theme of our work. The toll like receptor-7/8 activation by siRNA in order to boost the production of Interferon type -1 molecules to inhibit the viral and outer membrane LPS structure to activate Toll like receptor -4 to inhibit bacterial pathogens is the objective of this work. The delivery is made dependent on the peptide fragment which mediated the fusogenic mechanism so as to escape the endosomal compartment once endocytosed inside host(mamalian) cell. Thus freeing the siRNA to silence the myD88 transcript in host cytoplasm making RISC complex and hence, activating TLR-7/8 in endosomal membrane formerly.
 +
    Contact us: aitmnepal2013@gmail.com
 +
<br>
 +
<h2>Sponsors:</h2>
 +
<hr>
 +
<a href="http://www.cmdn.org.np/main/"><img src="https://static.igem.org/mediawiki/2013/7/78/Cmdnlogo.jpg"></a>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- Main Content Section Complete -->

Latest revision as of 20:07, 21 November 2014




Our Project:

ImmunEcoli

This is the first approach for Synthetic Biology from Nepal. We are a team of Undergrads of Biotechnology from Asian Institute of Technology and Management. This year we are trying to engineer ''Escherichia coli'' which will be used as a carrier and immune modulation point using Outer Membrane Vesicle (OMVs). ''Escherichia coli'', being a model organism, is easier to work with , especially for the purpose of genetically engineered realm. Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. The delivery of siRNA in a packaged outer membrane vesicle of gram negative bacteria is the theme of our work. The toll like receptor-7/8 activation by siRNA in order to boost the production of Interferon type -1 molecules to inhibit the viral and outer membrane LPS structure to activate Toll like receptor -4 to inhibit bacterial pathogens is the objective of this work. The delivery is made dependent on the peptide fragment which mediated the fusogenic mechanism so as to escape the endosomal compartment once endocytosed inside host(mamalian) cell. Thus freeing the siRNA to silence the myD88 transcript in host cytoplasm making RISC complex and hence, activating TLR-7/8 in endosomal membrane formerly. Contact us: aitmnepal2013@gmail.com

Sponsors:

Retrieved from "http://2013.igem.org/Team:AITM-Nepal"