Team:BostonU/Protocols

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<h2>Protocols</h2>
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<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
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We hope they help anyone interested in our experiments to successfully repeat them.
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<ul id="nav">
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<li><a href="#">Our Team</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Team">Team</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Social">Summer Fun</a></li>
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<li><a href="#">Project</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/MoCloChara">Introduction to MoClo and Characterization</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/QS">Quorum Sensing</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/HK">Histidine Kinase</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/ML">MoClo Library</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Results ">Results Summary</a></li>  
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<li><a href="#">Achievements</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Data">Data Collected</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>
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<li><a href="#">Notebook</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Clotho">Clotho and Eugene</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/NotebookQS">Quorum Sensing Notebook</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookHK">Histidine Kinase Notebook</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookML">MoClo Library Notebook</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/TroubleShooting">Trouble Shooting</a></li>
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<li><a href="#">Considerations</a>
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<li><a href="https://2013.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Safety">Safety</a></li>
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<li><a href="#">Acknowledgements</a>  
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                    <li><a href="https://2013.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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                    <li><a href="https://2013.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
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Latest revision as of 16:48, 16 August 2013



Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.

DNA Quantification
Gel Extraction
Miniprep
PCR Cleanup
Ligation
Media Prep
MoClo Level 0 Reaction
MoClo Level 1 and 2 Reactions
Phusion PCR
Restriction Digest
Restriction Digest 10 µL mapping
Transformation

Retrieved from "http://2013.igem.org/Team:BostonU/Protocols"