Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Dailylog

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(Difference between revisions)

Latest revision as of 13:33, 9 September 2013

Small Phage May - June Notebook: May 1 - May 12 Daily Log

Small Phage

5/1/13

- Commencement of spring!!!

- Discussed goals and outlined plans for spring term

5/2/13

- Designed primers for amplifying and sequencing phage capsid protein

- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly

5/3/13

- Performed agar test, focusing primarily on ×8

5.3 T7 phage selection method test

- Processed phage amplification

5.3 T7 phage amplification/purification

5/4/13

- Performed dilution series using stock 5.3 (-1 through -11)

- Started two 5mL overnights of BL21

5/5/13

- Spot test using stock 5.3 and its dilution series (from 5.4)

5.3 T7 phage amplification/purification

- Started liquid culture for purification team (at around noon)

1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)

- Started 5.5 amplification from a plaque test

5.5 Amplification from a plaque test

5/6/13

- Continued 5.3 T7 phage amplification/purification

- Performed liquid culture phage concentration test

5.6 T7+ Liquid Culture Phage Concentration Test

5/7/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7

5/8/13

- Went over procedure for applying mutagen and PCR with Dr. Grose

- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test

- Started two 5mL BL21 overnights

- Learned how to create LB plates

5/9/13

- Practiced with ×6 and ×8 top agar

5.9 T7 Selection Method Test #2

- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2

- Discussed plans and schedule for next week.

5/10/13

- Worked on Progress Report

5/12/13

- Started two 5mL overnight at around 6pm

@@ Line 1: / Line 1: @@
 {{TeamBYUProvo}}
-'''5/1/13'''
+<br>
+{| width="100%"
+| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: May 1 - May 12 Daily Log'''</font>
+<br>
+<br>
+|- valign="top"
+| style="width: 18%; background-color: transparent;"|
+<font color="#333399" size="3" font face="Calibri">
+<font size = "4">
+: <u> '''Small Phage''' </u> </font>
+: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
+: [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]]
+: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]]
+: [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]]
+</font>
+| style="width: 82%; background-color: transparent;"|
+<font face="Calibri" size="3">
+<font size="4"> '''5/1/13''' </font>
 - Commencement of spring!!!
@@ Line 7: / Line 38: @@
 - Discussed goals and outlined plans for spring term
-'''5/2/13'''
+<br>
+<font size="4"> '''5/2/13''' </font>
 - Designed primers for amplifying and sequencing phage capsid protein
@@ Line 13: / Line 46: @@
 - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
-'''5/3/13'''
+<br>
+<font size="4"> '''5/3/13''' </font>
 - Performed agar test, focusing primarily on ×8
@@ Line 23: / Line 58: @@
 : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]
-'''5/4/13'''
+<br>
+<font size="4"> '''5/4/13''' </font>
 - Performed dilution series using stock 5.3 (-1 through -11)
@@ Line 29: / Line 66: @@
 - Started two 5mL overnights of BL21
-'''5/5/13'''
+<br>
+<font size="4"> '''5/5/13''' </font>
 - Spot test using stock 5.3 and its dilution series (from 5.4)
-: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test||5.3 T7 phage amplification/purification]]
+: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]
 - Started liquid culture for purification team (at around noon)
@@ Line 41: / Line 80: @@
 - Started 5.5 amplification from a plaque test
-: [[5.5 Amplification from a plaque test]]
+: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.5_Amplification_from_a_plaque_test|5.5 Amplification from a plaque test]]
-'''5/6/13'''
+<br>
-- Continued [[5.3 T7 phage amplification/purification]]
+<font size="4"> '''5/6/13''' </font>
+- Continued [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 phage amplification/purification]]
 - Performed liquid culture phage concentration test
-: [[5.6 T7+ Liquid Culture Phage Concentration Test]]
+: [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test|5.6 T7+ Liquid Culture Phage Concentration Test]]
-'''5/7/13'''
+<br>
+<font size="4"> '''5/7/13''' </font>
 - Started two 5mL of E coli BL21 overnight
@@ Line 57: / Line 100: @@
 - Designed procedure for applying mutagen and selecting for T7
-'''5/8/13'''
+<br>
+<font size="4"> '''5/8/13''' </font>
 - Went over procedure for applying mutagen and PCR with Dr. Grose
-- Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]
+- Performed spot tests under [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test|5.6 T7+ Liquid Culture Phage Concentration Test]]
 - Started two 5mL BL21 overnights
@@ Line 67: / Line 112: @@
 - Learned how to create LB plates
-'''5/9/13'''
+<br>
+<font size="4"> '''5/9/13''' </font>
 - Practiced with ×6 and ×8 top agar
-: [[5.9 T7 selection method test #2]]
+: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9_T7_selection_method_test|5.9 T7 Selection Method Test #2]]
-- Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]]
+- Started [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9 T7+ Liquid Culture Phage Concentration Test|5.9 T7+ Liquid Culture Phage Concentration Test #2]]
 - Discussed plans and schedule for next week.
+<br>
+<font size="4"> '''5/10/13''' </font>
+- Worked on [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/PR|Progress Report]]
+<br>
+<font size="4"> '''5/12/13''' </font>
+- Started two 5mL overnight at around 6pm
+<br>
+</font>
+|}
 {{TeamBYUProvoFooter}}