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13/08/13
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==Making agar, chloroamphenicol and chloroamphenicol/IPTG plates== | ==Making agar, chloroamphenicol and chloroamphenicol/IPTG plates== | ||
| + | *One container of 400 ml Luria Agar was melted down in the microwave. This is enough to make 20 agar petri dishes. | ||
| + | *This was poured straight into 4 petri dishes to make agar plates | ||
| + | *The remaining agar had 0.64 ul of chloroamphenicol added to it (concentration = 24 ug/ml), and was mixed thoroughly | ||
| + | *The resulting solution was poured into 4 petri dishes, to make 4 chloroamphenicol agar plates | ||
| + | *The remaining agar solution had 1.5 ul of IPTG added to it (concentration = 24 ug/ml) | ||
| + | *This was mixed thoroughly, and then used to make up the remaining 12 chloroamphenicol/IPTG plates. | ||
| + | |||
| + | ==Agarose gel preparation== | ||
| + | |||
| + | *20 ml of 5 x TBE, 80 ml of distilled water and 0.8 g of agarose was added into a container. | ||
| + | *The solution was boiled for 3 minutes, 1 minute at a time. | ||
| + | *The solution was left to cool. | ||
| + | *5 ul of ethidium bromide was added to the solution. | ||
| + | *The solution was then poured into the gel plate and left to set for 30 minutes. | ||
Latest revision as of 15:52, 13 August 2013
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Making agar, chloroamphenicol and chloroamphenicol/IPTG plates
- One container of 400 ml Luria Agar was melted down in the microwave. This is enough to make 20 agar petri dishes.
- This was poured straight into 4 petri dishes to make agar plates
- The remaining agar had 0.64 ul of chloroamphenicol added to it (concentration = 24 ug/ml), and was mixed thoroughly
- The resulting solution was poured into 4 petri dishes, to make 4 chloroamphenicol agar plates
- The remaining agar solution had 1.5 ul of IPTG added to it (concentration = 24 ug/ml)
- This was mixed thoroughly, and then used to make up the remaining 12 chloroamphenicol/IPTG plates.
Agarose gel preparation
- 20 ml of 5 x TBE, 80 ml of distilled water and 0.8 g of agarose was added into a container.
- The solution was boiled for 3 minutes, 1 minute at a time.
- The solution was left to cool.
- 5 ul of ethidium bromide was added to the solution.
- The solution was then poured into the gel plate and left to set for 30 minutes.
