Team:MSOE Milwaukee/Modeling

From 2013.igem.org

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We chose to model the second bacteria in our system. Each enzyme was carefully researched to find experimental evidence of both the km and the vmax values. The following table shows the enzyme and the kinetic equation driving the turnover.  
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A large aspect of our project was to assess the feasibility of industrial use. The first portion was determining the theoretical enzyme activity and total turnover rate.  
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((Insert table here))
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<img align = center src="https://static.igem.org/mediawiki/2013/0/0d/Modeled_bioreactor.JPG" width = 960></img><br>
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After setting up a system using MATLAB (C) the final turnover rate was calculated based on the initial concentration of substrate and the inhibitors present in the system from different steps. We made the assumption that the system would be in a reactor at steady state and the following graph shows how the concentration of product will change based off of the concentration of substrate present at the inlet.
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After the total turnover rate was calculated, the next step was to design a bioreactor to carry out the reaction. A batch reactor was selected over a continuous reactor to best suit the bacterial life cycle. The governing equations of a bioreactor were used to calculate the concentration of the substrate, cells and product concentration. The inhibitory affect of eucalyptol on cell growth was found to be negligible when accounting for the bioreactor concentration. Preliminary cost assessment is shown in the table below.  
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Table showing the feasibility assessment of designing a plant capable of producing eucalyptol.
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  Reactor Size
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  Operating Cost
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  Product Cost
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  Current Market Cost
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  Profit at $10/kg
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  Payoff time
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  10,000L
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  ~$5million/yr
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  $5.51/kg
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  $64/kg
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  ~$4.6million/yr
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  4 years
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Latest revision as of 22:13, 22 September 2013

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A large aspect of our project was to assess the feasibility of industrial use. The first portion was determining the theoretical enzyme activity and total turnover rate.


After the total turnover rate was calculated, the next step was to design a bioreactor to carry out the reaction. A batch reactor was selected over a continuous reactor to best suit the bacterial life cycle. The governing equations of a bioreactor were used to calculate the concentration of the substrate, cells and product concentration. The inhibitory affect of eucalyptol on cell growth was found to be negligible when accounting for the bioreactor concentration. Preliminary cost assessment is shown in the table below.

Table showing the feasibility assessment of designing a plant capable of producing eucalyptol.

Retrieved from "http://2013.igem.org/Team:MSOE_Milwaukee/Modeling"
Reactor Size Operating Cost Product Cost Current Market Cost Profit at $10/kg Payoff time
10,000L ~$5million/yr $5.51/kg $64/kg ~$4.6million/yr 4 years