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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Dailylog
From 2013.igem.org
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| - | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: May 1 - May 12 Daily Log'''</font> |
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<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
| - | + | <font size = "4"> | |
| - | + | : <u> '''Small Phage''' </u> </font> | |
| - | [[Team:BYU Provo/Notebook/SmallPhage/ | + | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] |
| - | [[Team:BYU Provo/Notebook/SmallPhage/ | + | : [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]] |
| - | [[Team:BYU Provo/Notebook/SmallPhage/Fallexp| | + | : [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]] |
| + | |||
| + | : [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]] | ||
</font> | </font> | ||
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- Spot test using stock 5.3 and its dilution series (from 5.4) | - Spot test using stock 5.3 and its dilution series (from 5.4) | ||
| - | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 | + | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]] |
- Started liquid culture for purification team (at around noon) | - Started liquid culture for purification team (at around noon) | ||
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- Started 5.5 amplification from a plaque test | - Started 5.5 amplification from a plaque test | ||
| - | : [[5.5 Amplification from a plaque test]] | + | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.5_Amplification_from_a_plaque_test|5.5 Amplification from a plaque test]] |
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<font size="4"> '''5/6/13''' </font> | <font size="4"> '''5/6/13''' </font> | ||
| - | - Continued [[5.3 T7 phage amplification/purification]] | + | - Continued [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 phage amplification/purification]] |
- Performed liquid culture phage concentration test | - Performed liquid culture phage concentration test | ||
| - | : [[5.6 T7+ Liquid Culture Phage Concentration Test]] | + | : [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test|5.6 T7+ Liquid Culture Phage Concentration Test]] |
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- Went over procedure for applying mutagen and PCR with Dr. Grose | - Went over procedure for applying mutagen and PCR with Dr. Grose | ||
| - | - Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]] | + | - Performed spot tests under [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test|5.6 T7+ Liquid Culture Phage Concentration Test]] |
- Started two 5mL BL21 overnights | - Started two 5mL BL21 overnights | ||
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- Practiced with ×6 and ×8 top agar | - Practiced with ×6 and ×8 top agar | ||
| - | : [[5.9 T7 | + | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9_T7_selection_method_test|5.9 T7 Selection Method Test #2]] |
| - | - Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]] | + | - Started [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9 T7+ Liquid Culture Phage Concentration Test|5.9 T7+ Liquid Culture Phage Concentration Test #2]] |
- Discussed plans and schedule for next week. | - Discussed plans and schedule for next week. | ||
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| + | <br> | ||
| + | |||
| + | <font size="4"> '''5/10/13''' </font> | ||
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| + | - Worked on [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/PR|Progress Report]] | ||
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| + | <br> | ||
| + | |||
| + | <font size="4"> '''5/12/13''' </font> | ||
| + | |||
| + | - Started two 5mL overnight at around 6pm | ||
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| + | <br> | ||
</font> | </font> | ||
Latest revision as of 13:33, 9 September 2013
| Small Phage May - June Notebook: May 1 - May 12 Daily Log
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5/1/13 - Commencement of spring!!! - Discussed goals and outlined plans for spring term
5/2/13 - Designed primers for amplifying and sequencing phage capsid protein - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13 - Performed agar test, focusing primarily on ×8 - Processed phage amplification
5/4/13 - Performed dilution series using stock 5.3 (-1 through -11) - Started two 5mL overnights of BL21
5/5/13 - Spot test using stock 5.3 and its dilution series (from 5.4) - Started liquid culture for purification team (at around noon)
- Started 5.5 amplification from a plaque test
5/6/13 - Continued 5.3 T7 phage amplification/purification - Performed liquid culture phage concentration test
5/7/13 - Started two 5mL of E coli BL21 overnight - Designed procedure for applying mutagen and selecting for T7
5/8/13 - Went over procedure for applying mutagen and PCR with Dr. Grose - Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test - Started two 5mL BL21 overnights - Learned how to create LB plates
5/9/13 - Practiced with ×6 and ×8 top agar - Started 5.9 T7+ Liquid Culture Phage Concentration Test #2 - Discussed plans and schedule for next week.
5/10/13 - Worked on Progress Report
5/12/13 - Started two 5mL overnight at around 6pm
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