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Exeter/12 August 2013
From 2013.igem.org
(Difference between revisions)
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| + | {{:Team:Exeter/Template/Header}} | ||
| + | <div class="container"> | ||
| + | <div class="row"> | ||
| + | <div class="span" style="text-align:justify"> | ||
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Mini Preps of liquid cultures that worked | Mini Preps of liquid cultures that worked | ||
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| RBS + yellow (S05058) || 56.7 || 4.41 | | RBS + yellow (S05058) || 56.7 || 4.41 | ||
|- | |- | ||
| - | | | + | | ''ompC'' || 76.7 || 3.20 |
|- | |- | ||
| Promoter + RBS || 62.6 || 3.99 | | Promoter + RBS || 62.6 || 3.99 | ||
| Line 47: | Line 52: | ||
| Magenta diluted || 7.0 || 9.0 | | Magenta diluted || 7.0 || 9.0 | ||
|} | |} | ||
| + | |||
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! Culture !! DNA (pigment) + DNA (term) + DNA plasmid !! How much DNA is in there !! +T4 !! Water | ||
| + | |- | ||
| + | | Magenta || 0.648 + 0.12 + 2.0 || 2.768 || 4.268 || 5.732 | ||
| + | |- | ||
| + | | Yellow (K592010) || 0.664 + 0.12 + 2.0 || 2.784 || 4.284 || 5.716 | ||
| + | |- | ||
| + | | Yellow (S05058) || 0.680 + 0.12 + 2.0 || 2.80 || 4.30 || 5.70 | ||
| + | |- | ||
| + | | RFP || 0.992 + 0.12 + 2.0 || 3.112 || 4.612 || 5.388 | ||
| + | |} | ||
| + | |||
| + | |||
| + | ==Gel of digest== | ||
| + | |||
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | !Lane !! Content | ||
| + | |- | ||
| + | | 1 || 1kb Gene Ruler | ||
| + | |- | ||
| + | | 2 || Yellow (S05058) | ||
| + | |- | ||
| + | | 3 || Magenta | ||
| + | |- | ||
| + | | 4 || Yellow (K592010) | ||
| + | |- | ||
| + | | 5 || RFP | ||
| + | |- | ||
| + | | 6 || AMP plasmid | ||
| + | |- | ||
| + | | 7 || B0015 | ||
| + | |- | ||
| + | | 8 || 1kb Gene Ruler | ||
| + | |} | ||
| + | |||
| + | [[Image:12.8.13.jpg|center||500px|Image: 500 pixels]] | ||
| + | |||
| + | Unfortunately lanes 6 and 7 appear to have not worked. This may be due to human error, or a very low concentration of DNA being pipetted into the wells; the amount is insufficient to show a band. | ||
| + | |||
| + | Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | {{:Team:Exeter/Template/Footer}} | ||
Latest revision as of 20:40, 2 October 2013
Mini Preps of liquid cultures that worked
NanoDrop
| Culture | NanoDrop concentration (ng/ul) | ul to get 250ng |
|---|---|---|
| AMP plasmid | 60.0 | 4.17 |
| Yellow (K592010) | 49.1 | 5.09 |
| RBS + cph8 | 81.2 | 3.08 |
| Terminators | 27.9 | 8.96 |
| RFP control | 51.3 | 4.87 |
| RBS + yellow (S05058) | 56.7 | 4.41 |
| ompC | 76.7 | 3.20 |
| Promoter + RBS | 62.6 | 3.99 |
| Magenta | 368.1 | 0.67 |
| Magenta diluted | 35.7 | 7.0 |
| Culture | ul to get 250ng | 16ul - answer |
|---|---|---|
| AMP plasmid | 4.17 | 11.83 |
| Yellow (K592010) | 5.09 | 10.91 |
| B0015 | 8.96 | 7.04 |
| RFP | 4.87 | 11.13 |
| RBS + yellow (S05058) | 4.41 | 11.59 |
| Magenta diluted | 7.0 | 9.0 |
| Culture | DNA (pigment) + DNA (term) + DNA plasmid | How much DNA is in there | +T4 | Water |
|---|---|---|---|---|
| Magenta | 0.648 + 0.12 + 2.0 | 2.768 | 4.268 | 5.732 |
| Yellow (K592010) | 0.664 + 0.12 + 2.0 | 2.784 | 4.284 | 5.716 |
| Yellow (S05058) | 0.680 + 0.12 + 2.0 | 2.80 | 4.30 | 5.70 |
| RFP | 0.992 + 0.12 + 2.0 | 3.112 | 4.612 | 5.388 |
Gel of digest
| Lane | Content |
|---|---|
| 1 | 1kb Gene Ruler |
| 2 | Yellow (S05058) |
| 3 | Magenta |
| 4 | Yellow (K592010) |
| 5 | RFP |
| 6 | AMP plasmid |
| 7 | B0015 |
| 8 | 1kb Gene Ruler |
Unfortunately lanes 6 and 7 appear to have not worked. This may be due to human error, or a very low concentration of DNA being pipetted into the wells; the amount is insufficient to show a band.
Take me back to the notebook.
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