Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/PR
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- | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: May 13 - May 26 Progress Report'''</font> |
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- | : | + | : <u> '''Small Phage''' </u> </font> |
- | : [[Team:BYU Provo/Notebook/SmallPhage/ | + | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] |
- | : [[Team:BYU Provo/Notebook/SmallPhage/ | + | : [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]] |
- | : [[Team:BYU Provo/Notebook/SmallPhage/Fallexp| | + | : [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]] |
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+ | : [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]] | ||
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5.20 Mutagen Concentration Experiment | 5.20 Mutagen Concentration Experiment | ||
- | : In order to determine the best concentration of mutagen to use, we infected the E. coli in 5 tubes with 0ul, 10ul, 50ul, 100ul, and 200ul of our mutagen, 5-bromodeoxyuridine. We then added 6uL of 5.3 phage stock to each tube, allowed it to incubate for 20 minutes, and purified the phage. Next, we made a dilution series and performed a spot test and found that when the mutagen concentration is increased, the concentration of phage is decreased. | + | : In order to determine the best concentration of mutagen to use, we infected the E. coli in 5 tubes with 0ul, 10ul, 50ul, 100ul, and 200ul of our mutagen, 5-bromodeoxyuridine. We then added 6uL of 5.3 phage stock to each tube, allowed it to incubate for 20 minutes, and purified the phage. Next, we made a dilution series and performed a spot test and found that when the mutagen concentration is increased, the concentration of phage is decreased. |
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+ | [[File:MutagenPlate1.JPG|400px|center]] | ||
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+ | We then performed titers from -6, -7, and -8 of phage from our dilution series with x8 top agar. There are a couple plaques on each plate. | ||
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[[File:MutagenPlate3.JPG|400px|center]] | [[File:MutagenPlate3.JPG|400px|center]] |
Latest revision as of 13:43, 9 September 2013
Small Phage May - June Notebook: May 13 - May 26 Progress Report
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1. Goals for the week
5.9 T7+ Liquid Culture Phage Concentration Test #2
5.13 Determining E. coli Concentration With Spectrophotometer
5.15 Titer Test on 5.3 T7 new Phage Stock
5.20 Mutagen Concentration Experiment
We then performed titers from -6, -7, and -8 of phage from our dilution series with x8 top agar. There are a couple plaques on each plate.
5.20 T7 Minor Capsid Protein PCR
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