"

From 2013.igem.org

Revision as of 14:14, 22 August 2013 (view source) AliceHaworth (Talk | contribs) (→Single digest) ← Older edit		Latest revision as of 14:16, 22 August 2013 (view source) AliceHaworth (Talk | contribs) (→Single digest)
(One intermediate revision not shown)
Line 17:		Line 17:
	*Buffer used was cutsmart		*Buffer used was cutsmart
	*Incubate samples at 37C for 1h		*Incubate samples at 37C for 1h
-	*Heat kill for 5min at	+	*2ul of orange G dye was added to each samples
		+	*Samples were then frozen overnight to run on gel on Monday

---

Latest revision as of 14:16, 22 August 2013

Single digest

• Digesting RFP plasmid again using a single restriction enzyme- XbaI
• Digesting DNA samples 1-6
• 200ng of DNA is run
• Volumes for digest:

Sample nr	1	2	3	4	5	6
DNA	0.7	0.7	0.8	1.3	1.2	2.6
Buffer	2	2	2	2	2	2
Water	16.3	16.3	16.2	15.7	15.8	14.4
XbaI	1	1	1	1	1	1

• Buffer used was cutsmart
• Incubate samples at 37C for 1h
• 2ul of orange G dye was added to each samples
• Samples were then frozen overnight to run on gel on Monday