Team:BYU Provo/Notebook/SmallPhage/Fallexp

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<font size="5" font face="Calibri"> '''Sep 1 - July 15''' </font>
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<font size="5" font face="Calibri"> '''September 1 - September 15''' </font>
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<font size="3" font face="Calibri"> A new term and a fresh start! During these two weeks, we performed two more rounds of mutagenesis. With every experiment, we were able to perfect our protocol. Although our results are still not satisfactory, we are getting close! Eventually, we discovered that our phage stock decreased in titer during its preservation inside the fridge. We will propagate our phage and try again!</font>
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<font size="3" font face="Calibri"> While the phage purification team was working out a kink in their cesium chloride gradient, we focused on modeling the relationship between capsid size and plaque size. Then once the cesium chloride gradient was perfected, we performed another round of mutagenesis, hoping that we would now be able to see mutant T7 bacteriophage. </font>
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[[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/Period1/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period1/Explist|Experiment Listing]]
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| <font size="5" font face="Calibri"> '''Sep 16 - Sep 30''' </font>
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| <font size="5" font face="Calibri"> '''September 16 - September 30''' </font>
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<font size="3" font face="Calibri"> During these couple weeks, we tried another 2 rounds of mutagenesis. The 5th round didn't work well so we made some major adjustments for the 6th round of mutagenesis. We believe that we were successful in finally mutating the phage and are giving them to the phage purification team to run a cesium chloride gradient on them. </font>
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<font size="3" font face="Calibri"> All our hard work finally paid off! We managed to isolate a smaller and a larger T7 bacteriophage! </font>
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[[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/Period2/Explist|Experiment Listing]]
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| <font size="5" font face="Calibri"> '''October 1 - October 15''' </font>
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<font size="3" font face="Calibri"> We had a great time at the iGEM regionals in Toronto! We're excited to be going to the World Championship Jamboree and got right back to work. Our first focus was getting good pictures of our mutant phage so that we could confirm their phenotypes. </font>
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period3/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period3/Explist|Experiment Listing]]
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| <font size="5" font face="Calibri"> '''October 16 - October 31''' </font>
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<font size="3" font face="Calibri"> For the last weeks of iGEM, we continued to characterize the mutant phage more. We sequenced the capsid protein and searched for mutations. In addition, we cloned in the capsid proteins for T7 wild type, S4 (Small mutant 1), S10 (Small mutant 2), and L8 (Large Mutant 1).  </font>
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<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period4/Explist|Experiment Listing]]
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{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 03:27, 29 October 2013


Small Phage September - October Notebook



Small Phage
March-April
May-June
July-August
September-October

September 1 - September 15


While the phage purification team was working out a kink in their cesium chloride gradient, we focused on modeling the relationship between capsid size and plaque size. Then once the cesium chloride gradient was perfected, we performed another round of mutagenesis, hoping that we would now be able to see mutant T7 bacteriophage.


Daily log

Experiment Listing


Asdfsa.JPG


September 16 - September 30


All our hard work finally paid off! We managed to isolate a smaller and a larger T7 bacteriophage!


Daily log


BYUSPFall2.JPG
October 1 - October 15


We had a great time at the iGEM regionals in Toronto! We're excited to be going to the World Championship Jamboree and got right back to work. Our first focus was getting good pictures of our mutant phage so that we could confirm their phenotypes.


Daily log

Experiment Listing


File:Asdfsa1.JPG


October 16 - October 31


For the last weeks of iGEM, we continued to characterize the mutant phage more. We sequenced the capsid protein and searched for mutations. In addition, we cloned in the capsid proteins for T7 wild type, S4 (Small mutant 1), S10 (Small mutant 2), and L8 (Large Mutant 1).


Daily log

Experiment Listing


220px

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