Exeter/3 September 2013

From 2013.igem.org

(Difference between revisions)
Line 82: Line 82:
! Part !! Concentration (ng/ul)
! Part !! Concentration (ng/ul)
|-
|-
-
| CcaR ||324.6
+
| gBlock 1 ||324.6
|-
|-
-
| CcaS || 264.4
+
| gBlock 2 || 264.4
|-
|-
-
| Yellow || 64.3
+
| gBlock 3 || 64.3
|-
|-
-
| Magenta || 102.7
+
| gBlock 4 || 102.7
|-
|-
-
| RBS || 48.9
+
| gBlock 5 || 48.9
|-
|-
-
| Promoter, RBS, #1 || 21.5
+
| gBlock 6 || 21.5
|-
|-
-
| Promoter, RBS, #2 || 22.6
+
| gBlock 7 || 22.6
|-
|-
-
| Promoter, RBS, #3 || 22.0
+
| gBlock 8 || 22.0
|-
|-
-
| Fix L || 31.9
+
| gBlock 9 || 31.9
|-
|-
-
| Fix J || 35.4
+
| gBlock 10 || 35.4
|-
|-
-
| YF1 || 27.1
+
| gBlock 11 || 27.1
|-
|-
-
| OmpF || 28.2
+
| gBlock 12 || 28.2
 +
|-
 +
| gBlock 13 || 31.9
 +
|-
 +
| gBlock 14 || 35.4
 +
|-
 +
| gBlock 15 || 27.1
 +
|-
 +
| OmpF + RBS A || 35.4
 +
|-
 +
| OmpF + RBS B || 27.1
 +
|-
 +
| OmpF + RBS C || 28.2
 +
|-
 +
| OmpF + RBS D || 31.9
 +
|-
 +
| K592011 B|| 35.4
|}
|}

Revision as of 14:46, 3 September 2013

Contents

Results of liquid cultures from yesterday

gBlocks

colours of our liquid cultures
gBlock Colour
1 No
2 No
3 Vibrant pink
4 No
5 Vibrant pink
6 No
7 Peach
8 No
9 Pink - not so vibrant
10 Vibrant pink
11 Vibrant pink
12 Pink - not so vibrant
13 Pink - not so vibrant
14 No
15 No


Part Did it work?
OmpF + RBS A ✓
OmpF + RBS B ✓
OmpF + RBS C ✓
OmpF + RBS D ✓
K592022 A ✗
K592022 B ✓
K592011 A ✗
K592011 B ✓
K864404 ✓


Miniprepped

  • OmpF + RBS A
  • OmpF + RBS B
  • OmpF + RBS C
  • OmpF + RBS D
  • K592011 B
  • gblocks 1 to 15

NanoDrop from miniprep

Part Concentration (ng/ul)
gBlock 1 324.6
gBlock 2 264.4
gBlock 3 64.3
gBlock 4 102.7
gBlock 5 48.9
gBlock 6 21.5
gBlock 7 22.6
gBlock 8 22.0
gBlock 9 31.9
gBlock 10 35.4
gBlock 11 27.1
gBlock 12 28.2
gBlock 13 31.9
gBlock 14 35.4
gBlock 15 27.1
OmpF + RBS A 35.4
OmpF + RBS B 27.1
OmpF + RBS C 28.2
OmpF + RBS D 31.9
K592011 B 35.4
Retrieved from "http://2013.igem.org/Exeter/3_September_2013"