Introduction

Synthetic biology doesn’t stop at the level of molecular systems. To expand the scope of this project we have chosen for a multi-level approach, in which we are working on biobricks, proteins, a pathway and also our host. In order to achieve the latter two strategies have been conceived of. In the first we have chosen to harness the power of directed evolution, a powerful tool that not often used in this competition. An explanation for this might lie in the fact that this approach is only semi-rational at best, however we like to argue that this does not make it any less of a powerful mechanism, and neither within the field of synthetic biology. Nonetheless, in order to explore new territories we have chosen for a second, fully rational approach in which we analyze the transcriptome of two distinct phenotypes; the mycelial and the single cell.

Abstract

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