Team:UNITN-Trento/Notebook/Labposts/06/34

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Latest revision as of 07:49, 3 October 2013

{ "date" : "2013-06-17", "author" : "thomas", "title" : "Cloning of EFE in pSB1C3", "content" : "I began the week doing some minipreps (x5 of EFE and x5 of pSB1C3+RFP) following this protocol.

Quantification results

Sample	EFE	pSB1C3
1	253,8 ng/ul	161,9 ng/ul
1	243,5 ng/ul	160,9 ng/ul
3	261,4 ng/ul	142,5 ng/ul
4	218,0 ng/ul	168,4 ng/ul
5	299,1 ng/ul	sample lost

I then digested the linearized pSB1C3 (500 ng, kindly offered by Caterina), pSB1C3 + RFP (500 ng) and EFE (1000 ng) with EcorI and PstI following the 2A assembly protocol.After that, I proceeded with the ligation and transformation in NEB10B cells. ", "tags" : "EFE" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-18",
+	"date" : "2013-06-17",
-	"author" : "fabio-bruno",
+	"author" : "thomas",
-	"title" : "bacillus subtilis promoters (part3)!!",
+	"title" : "Cloning of EFE in pSB1C3",
-	"content" : "<html> from the previous inoculum ( with NEB10b cells) we could only take K823000 ( duplicate) and K143012 (triplicate). Then we performed the minipreps and quantified the yields:K823000 a = 30.7 ng/ulK823000 b = 34.2 ng/ulK143012a = 26.8 ng/ulK143012b = 30.3 ng/ulK143012c = 29.5 ng/ulAs far as NEB5a are concerned, from the inoculum we made our cells competent.In the afternoon we transformed these cells with our promoters.</html>",
+	"content" : "I began the week doing some minipreps (x5 of EFE and x5 of pSB1C3+RFP) following <html><a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">this protocol</a></html>. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>EFE</th><th>pSB1C3</th></tr><tr><td>1</td><td>253,8 ng/ul</td><td>161,9 ng/ul</td></tr><tr><td>1</td><td>243,5 ng/ul</td><td>160,9 ng/ul</td></tr><tr><td>3</td><td>261,4 ng/ul</td><td>142,5 ng/ul</td></tr><tr><td>4</td><td>218,0 ng/ul</td><td>168,4 ng/ul</td></tr><tr><td>5</td><td>299,1 ng/ul</td>                <td>sample lost</td></tr></table></center></html>}}I then <html><a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digested</a></html> the linearized pSB1C3 (500 ng, kindly offered by Caterina), pSB1C3 + RFP (500 ng) and EFE (1000 ng) with EcorI and PstI following the 2A assembly protocol.After that, I proceeded with the <html><a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\">ligation</a></html> and <html><a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation</a></html> in NEB10B cells.  ",
-	"tags" : "Pveg-PliaG-PlepA"
+	"tags" : "EFE"
 }