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Team:DTU-Denmark/Notebook/9 September 2013
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*negative Nir in pZA21::ara tight - no colonies | *negative Nir in pZA21::ara tight - no colonies | ||
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Revision as of 15:31, 9 September 2013
9 September
Contents |
lab 208
Main purpose
Who was in the lab
Julia, Kristian, Henrike
Procedure
Colony PCR to verify inserts HAO and Nir in pZA21::ara and pZA21::ara tight
Results
Transfomation
Transformation on last friday gave the following results:
- HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
- negative HAO in pZA21::ara (from Biobrick K808000) - no colonies
- HAO in pZA21::ara tight - a lot of colonies, some very big
- negative HAO in pZA21::ara tight - a lot of small colonies
- Nir in pZA21::ara tight - 8 colonies
- negative Nir in pZA21::ara tight - no colonies
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