Team:UCLA

From 2013.igem.org

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<h1>UCLA's debut iGEM team!</h1>
 
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<p><b>DiversiPhage: Library Generation for Protein Selection</b></p>
 
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<p>Both the mammalian immune system’s complex defenses and a bacteriophage’s targeting mechanism depend upon protein diversification. These models have inspired innovations ranging from targeted drug delivery to protein display. Using the major tropism determining (MTD) protein expressed on the Bordatella bacteriophage BPP-1, we aim to develop an in vitro system for generating antibody-like proteins that bind specified targets. The MTD protein expressed at the phage’s tail fiber is naturally modified at its variable region to produce nearly 10<sup>13</sup> possible binding variants while preserving its structure. Mutating the MTD’s variable region by PCR can match the massive diversity of MTD in vitro. A library of MTD protein-DNA fusions generated by mRNA display can then be screened for binding against specified protein targets. This in vitro analog to phage display and immune clonal selection can be a powerful tool for constructing target-binding MTD variants with equally many varied applications.
 
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<p>As members of UCLA's Synthetic Biology Club and iGEM team, we are excited to see where the field of synthetic biology, and the iGEM competition, can take us!</p>
 
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=UCLA's debut iGEM team!=
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<!--- The Mission, Experiments --->
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===DiversiPhage: Library Generation for Protein Selection===
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Both the mammalian immune system’s complex defenses and a bacteriophage’s targeting mechanism depend upon protein diversification. These models have inspired innovations ranging from targeted drug delivery to protein display. Using the major tropism determining (MTD) protein expressed on the Bordatella bacteriophage BPP-1, we aim to develop an in vitro system for generating antibody-like proteins that bind specified targets. The MTD protein expressed at the phage’s tail fiber is naturally modified at its variable region to produce nearly 10<sup>13</sup> possible binding variants while preserving its structure. Mutating the MTD’s variable region by PCR can match the massive diversity of MTD in vitro. A library of MTD protein-DNA fusions generated by mRNA display can then be screened for binding against specified protein targets. This in vitro analog to phage display and immune clonal selection can be a powerful tool for constructing target-binding MTD variants with equally many varied applications.
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!align="center"|[[Team:UCLA|Home]]
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!align="center"|[[Team:UCLA/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=UCLA Official Team Profile]
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<p>As members of UCLA's Synthetic Biology Club and iGEM team, we are excited to see where the field of synthetic biology, and the iGEM competition, can take us!</p>
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!align="center"|[[Team:UCLA/Project|Project]]
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!align="center"|[[Team:UCLA/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UCLA/Modeling|Modeling]]
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!align="center"|[[Team:UCLA/Notebook|Notebook]]
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!align="center"|[[Team:UCLA/Safety|Safety]]
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!align="center"|[[Team:UCLA/Attributions|Attributions]]
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Revision as of 01:37, 11 September 2013



UCLA's debut iGEM team!

DiversiPhage: Library Generation for Protein Selection

Both the mammalian immune system’s complex defenses and a bacteriophage’s targeting mechanism depend upon protein diversification. These models have inspired innovations ranging from targeted drug delivery to protein display. Using the major tropism determining (MTD) protein expressed on the Bordatella bacteriophage BPP-1, we aim to develop an in vitro system for generating antibody-like proteins that bind specified targets. The MTD protein expressed at the phage’s tail fiber is naturally modified at its variable region to produce nearly 1013 possible binding variants while preserving its structure. Mutating the MTD’s variable region by PCR can match the massive diversity of MTD in vitro. A library of MTD protein-DNA fusions generated by mRNA display can then be screened for binding against specified protein targets. This in vitro analog to phage display and immune clonal selection can be a powerful tool for constructing target-binding MTD variants with equally many varied applications.


As members of UCLA's Synthetic Biology Club and iGEM team, we are excited to see where the field of synthetic biology, and the iGEM competition, can take us!

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