Exeter/12 September 2013

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==Optical Absorption of liquid cultures==
==Optical Absorption of liquid cultures==
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In the afternoon we transformed the ligations from earlier in the week.  This was also done on Tuesday, however, we now have fresh competent cells that we used instead.
In the afternoon we transformed the ligations from earlier in the week.  This was also done on Tuesday, however, we now have fresh competent cells that we used instead.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Latest revision as of 07:44, 30 September 2013

Exeter iGEM 2013 · Paint by Coli

Optical Absorption of liquid cultures

Using the liquid cultures that were made yesterday afternoon, today we used a plate reader to measure the absorbance of the cyan and magenta cultures. Cyan was measured every 4 hours as the colour appears to be developing much slower than the others, but the magenta was measured every two hours.


Liquid culture Absorbance at 10:00am Absorbance at 12:00pm Absorbance at 14:00pm Absorbance at 16:00pm
Cyan no.2 0.602 -  ? -
Cyan no.3 0.817 -  ? -
Cyan V.2 0.894 -  ? -
Cyan V.4 0.740 -  ? -
G block 11 0.542 0.519  ?  ?
G block 7 1.023 1.120  ?  ?

Transformations

In the afternoon we transformed the ligations from earlier in the week. This was also done on Tuesday, however, we now have fresh competent cells that we used instead.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/12_September_2013"