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12/09/13
From 2013.igem.org
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*Pellet resuspended in 375ul of HMFM | *Pellet resuspended in 375ul of HMFM | ||
*Samples were then frozen at -80 | *Samples were then frozen at -80 | ||
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| + | ==Gel Purification of the pGEM-T pasmid double digestions ran in the [[11/09/13]]== | ||
Revision as of 20:09, 13 September 2013
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Contents |
Isolate plasmid from overnight culture
- Using Omega mini prep kit protocol
- Samples:
- TODF 1,2,3,
- TODX 1, 2, 3
- TOBG 1, 2, 3,
- RFP 1, 2, 3
| sample | Volume (ul) | Concentration (ng/ul) |
| TODF1 | 43 | 120 |
| TODF2 | 43 | 98.8 |
| TODX1 | 42 | 399.3 |
| TODX2 | 44 | 442.2 |
| TODX3 | 43 | 417.5 |
| TOBG1 | 43 | 262.4 |
| TOBG2 | 47 | 319.2 |
| TOBG3 | 41 | 338.4 |
| RFP1 | 41 | 77.7 |
| RFP2 | 41 | 32.7 |
| RFP3 | 42 | 59 |
Glycerol stocks
- Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
- Supernatant was removed
- Pellet resuspended in 375ul of HMFM
- Samples were then frozen at -80
Gel Purification of the pGEM-T pasmid double digestions ran in the 11/09/13
Double Digest
