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Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog
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Clarice has been working on submitting parts to the registry. She has cloned the Ydiv gene into the iGEM backbone to submit the part; we purified the plasmid for submission. | Clarice has been working on submitting parts to the registry. She has cloned the Ydiv gene into the iGEM backbone to submit the part; we purified the plasmid for submission. | ||
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Revision as of 22:34, 16 September 2013
| Cholera Detection September-October Notebook: September 16 - September 29 Daily Log
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9/16/13 On Saturday we checked our bacteriophage spot test for plaques, but we saw none. Our bacteriophage lambda purification procedure failed. Dr. Grose thinks that inducing lambda to lysis by 43 degree heat shock must not have been sufficient stress. Since we know H202 does induce our phage, we're going to attempt to isolate bacteriophage lambda by doing a top agar plaque assay with a drop of hydrogen peroxide, then bathing the top agar in LB, sucking it all up and then purifying the bacteriophage by chloroform lysis procedure. Clarice has been working on submitting parts to the registry. She has cloned the Ydiv gene into the iGEM backbone to submit the part; we purified the plasmid for submission. KK, KP, CH
9/18/13
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