Team:KU Leuven/Project/Ecological/wetlab

From 2013.igem.org

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  <h3>E. coligy: Plants with BanAphids - insect experiments</h3>
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  <p align = "justify">Essentially, our project aims to offer plants a beneficial protection mechanism against aphids. Our <B>BanAphids</B>, the genetically modified <I>E. coli</I> strain, will provide plants with a protection mechanism by the production of two substances, methyl salicylate (MeS) and E-beta-farnesene (EBF), a phytohormone and a pheromone, respectively. MeS will activate plant defence systems and attract natural predators of aphids. Furthermore, EBF will repel aphids off of the plant and will attract natural predators of aphids. These two substances are naturally used in the communication between plants, predators and aphids. Therefore our <B>BanAphids</B> will blend into the ecosystem and minimise the disruption of the communication between plants, predators and aphids. Below, you will find more information concerning the effects of MeS and EBF on plants, predators and aphids.</p>
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  <img src="https://static.igem.org/mediawiki/2013/0/09/Aphidinfestedrose.png"/>
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  <p><B>Aphid infested Rose plant</B> (Mauro Mandrioli)</p>
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  <h3>BanAphid's products</h3>
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    <a href="#Methyl Salicylate"><h4>Methyl Salicylate</h4></a>
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    <a href="#MeS effect on aphids"><p>Aphids</p></a>
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    <a href="#MeS effect on predators"><p>Predators</p></a>
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    <a href="#MeS effect on plants1"><p>Plants-defence systems</p></a>
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    <a href="#MeS effect on plants2"><p>Plants-optimal defence theory</p></a>
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    <a href="#MeS effect on plants3"><p>Plants-above vs. below ground herbivory</p></a>
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    <a href="#E-beta-farnesene"><h4>E-beta-farnesene</h4></a>
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    <a href="#EBF effect on aphids"><p>Aphids - repellent</p></a>
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    <a href="#EBF effect on predators"><p>Predators - prey localisation </p></a>
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  <a href="#References"><h4>References</h4></a>
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Revision as of 18:07, 2 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon



Essentially, our project aims to offer plants a beneficial protection mechanism against aphids. Our BanAphids, the genetically modified E. coli strain, will provide plants with a protection mechanism by the production of two substances, methyl salicylate (MeS) and E-beta-farnesene (EBF), a phytohormone and a pheromone, respectively. MeS will activate plant defence systems and attract natural predators of aphids. Furthermore, EBF will repel aphids off of the plant and will attract natural predators of aphids. These two substances are naturally used in the communication between plants, predators and aphids. Therefore our BanAphids will blend into the ecosystem and minimise the disruption of the communication between plants, predators and aphids. Below, you will find more information concerning the effects of MeS and EBF on plants, predators and aphids.

  <img src="Aphidinfestedrose.png"/>

Aphid infested Rose plant (Mauro Mandrioli)

BanAphid's products

<a href="#Methyl Salicylate">

Methyl Salicylate

</a> <a href="#MeS effect on aphids">

Aphids

</a> <a href="#MeS effect on predators">

Predators

</a> <a href="#MeS effect on plants1">

Plants-defence systems

</a> <a href="#MeS effect on plants2">

Plants-optimal defence theory

</a> <a href="#MeS effect on plants3">

Plants-above vs. below ground herbivory

</a>
<a href="#E-beta-farnesene">

E-beta-farnesene

</a> <a href="#EBF effect on aphids">

Aphids - repellent

</a> <a href="#EBF effect on predators">

Predators - prey localisation

</a>
<a href="#References">

References

</a>