Template:Kyoto/Notebook/Aug 26

From 2013.igem.org

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</div>
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===Ligation===
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<!-- こっから -->
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<div class="experiment">
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<span class="author">Ashida</span>
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{| class="wikitable"
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!Name||-?-
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|-
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|8/21 DT||5.0
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|-
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|8/26 tetR aptamer 12_1R||2.0
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|-
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|Ligation High buffer||3.5
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|}
 +
{| class="wikitable"
 +
!Name||-?-
 +
|-
 +
|8/21 DT||5.0
 +
|-
 +
|8/22 pT181 atteniator||5.0
 +
|-
 +
|Ligation High buffer||5.0
 +
|}
 +
{| class="wikitable"
 +
!Name||-?-
 +
|-
 +
|8/21 DT||5.0
 +
|-
 +
|8/22 pT181 antisense||3.0
 +
|-
 +
|Ligation High buffer||4.0
 +
|}
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{| class="wikitable"
 +
!Name||-?-
 +
|-
 +
|8/21 DT||5.0
 +
|-
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|8/22 Spinach||3.0
 +
|-
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|Ligation High buffer||4.0
 +
|}
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{| class="wikitable"
 +
!Name||-?-
 +
|-
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|8/22 Plac||4.0
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|-
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|8/22 pT181 attenuator||5.0
 +
|-
 +
|Ligation High buffer||4.5
 +
|}
 +
{| class="wikitable"
 +
!Name||-?-
 +
|-
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|8/22 Pcon||5.0
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|-
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|8/22 pT181 attenuator||5.0
 +
|-
 +
|Ligation High buffer||5.0
 +
|}
 +
{| class="wikitable"
 +
!Name||-?-
 +
|-
 +
|8/22 Plac||4.0
 +
|-
 +
|8/22 pT181 antisense||8.0
 +
|-
 +
|Ligation High buffer||6.0
 +
|}
 +
{| class="wikitable"
 +
!Name||-?-
 +
|-
 +
|8/22 Pcon||4.0
 +
|-
 +
|8/22 pT181 antisense||8.0
 +
|-
 +
|Ligation High buffer||6.0
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->
 +
===Transformation===
===Transformation===
<!-- こっから -->
<!-- こっから -->

Revision as of 03:00, 19 September 2013

Contents

Aug 26

Gel Extraction

Matsumoto and Ashida

LaneDNAEnzyme
1100bp ladder-
28/24 tetR aptamer 12_PEcoRI&SpeI
48/24 tetR aptamer 12_1REcoRI&SpeI
68/24 tetR aptamer 12_MEcoRI&SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
tetR aptamer 12_P(EcoRI&SpeI)2.30.380.13
tetR aptamer 12_1R(EcoRI&SpeI)12.00.631.19
tetR aptamer 12_M(EcoRI&SpeI)2.06.430.03

Ligation

Ashida

Name-?-
8/21 DT5.0
8/26 tetR aptamer 12_1R2.0
Ligation High buffer3.5
Name-?-
8/21 DT5.0
8/22 pT181 atteniator5.0
Ligation High buffer5.0
Name-?-
8/21 DT5.0
8/22 pT181 antisense3.0
Ligation High buffer4.0
Name-?-
8/21 DT5.0
8/22 Spinach3.0
Ligation High buffer4.0
Name-?-
8/22 Plac4.0
8/22 pT181 attenuator5.0
Ligation High buffer4.5
Name-?-
8/22 Pcon5.0
8/22 pT181 attenuator5.0
Ligation High buffer5.0
Name-?-
8/22 Plac4.0
8/22 pT181 antisense8.0
Ligation High buffer6.0
Name-?-
8/22 Pcon4.0
8/22 pT181 antisense8.0
Ligation High buffer6.0

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
8/26 tetR aptamer 12_1R-DT1µL10µL11µLCP
8/26 pT181 attenuator-DT1µL10µL11µLCP
8/26 pT181 antisense-DT1µL10µL11µLCP
8/26 Spinach-DT1µL10µL11µLCP
8/26 Plac-pT181 attenuator1µL10µL11µLCP
8/26 Pcon-pT181 attenuator1µL10µL11µLAmp
8/26 Plac-pT181 antisense1µL10µL11µLCP
8/26 Pcon-pT181 antisense1µL10µL11µLAmp
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