Template:Kyoto/Notebook/Aug 26

From 2013.igem.org

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|8/26 Pcon-pT181 antisense||1µL||10µL||11µL||Amp
|8/26 Pcon-pT181 antisense||1µL||10µL||11µL||Amp
 +
|Pλ-RBS-luxI-DT||1µL||10µL||11µ||Amp
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Revision as of 04:42, 19 September 2013

Contents

Aug 26

Gel Extraction

Matsumoto and Ashida

LaneDNAEnzyme
1100bp ladder-
28/24 tetR aptamer 12_PEcoRI&SpeI
48/24 tetR aptamer 12_1REcoRI&SpeI
68/24 tetR aptamer 12_MEcoRI&SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
tetR aptamer 12_P(EcoRI&SpeI)2.30.380.13
tetR aptamer 12_1R(EcoRI&SpeI)12.00.631.19
tetR aptamer 12_M(EcoRI&SpeI)2.06.430.03

Ligation

Ashida

Name-?-
8/21 DT5.0
8/26 tetR aptamer 12_1R2.0
Ligation High buffer3.5
Name-?-
8/21 DT5.0
8/22 pT181 atteniator5.0
Ligation High buffer5.0
Name-?-
8/21 DT5.0
8/22 pT181 antisense3.0
Ligation High buffer4.0
Name-?-
8/21 DT5.0
8/22 Spinach3.0
Ligation High buffer4.0
Name-?-
8/22 Plac4.0
8/22 pT181 attenuator5.0
Ligation High buffer4.5
Name-?-
8/22 Pcon5.0
8/22 pT181 attenuator5.0
Ligation High buffer5.0
Name-?-
8/22 Plac4.0
8/22 pT181 antisense8.0
Ligation High buffer6.0
Name-?-
8/22 Pcon4.0
8/22 pT181 antisense8.0
Ligation High buffer6.0

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
8/26 tetR aptamer 12_1R-DT1µL10µL11µLCP
8/26 pT181 attenuator-DT1µL10µL11µLCP
8/26 pT181 antisense-DT1µL10µL11µLCP
8/26 Spinach-DT1µL10µL11µLCP
8/26 Plac-pT181 attenuator1µL10µL11µLCP
8/26 Pcon-pT181 attenuator1µL10µL11µLAmp
8/26 Plac-pT181 antisense1µL10µL11µLCP
8/26 Pcon-pT181 antisense1µL10µL11µLAmp Pλ-RBS-luxI-DT1µL10µL11µAmp
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