"

From 2013.igem.org

Revision as of 05:28, 19 September 2013 (view source) Nato (Talk | contribs) (→Transformation) ← Older edit		Revision as of 14:25, 19 September 2013 (view source) Mitsuaki (Talk | contribs) (→Ligation) Newer edit →
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	</div>		</div>
	===Ligation===		===Ligation===
-	<!-- こっから -->
	<div class="experiment">		<div class="experiment">
	<span class="author">Ashida</span>		<span class="author">Ashida</span>
	{| class="wikitable"		{| class="wikitable"
-	!Name||-?-	+	!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
	|-		|-
-	|8/21 DT||5.0	+	|experiment||8/21 DT||5.0||8/26 tetR aptamer 12_1R||2.0||3.5
	|-		|-
-	|8/26 tetR aptamer 12_1R||2.0	+	|experiment||8/21 DT||5.0||8/22 pT181 atteniator||5.0||5.0
	|-		|-
-	|Ligation High buffer||3.5	+	|experiment||8/21 DT||5.0||8/22 pT181 antisense||3.0||4.0
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
	|-		|-
-	|8/21 DT||5.0	+	|experiment||8/21 DT||5.0||8/22 Spinach||3.0||4.0
	|-		|-
-	|8/22 pT181 atteniator||5.0	+	|experiment||8/22 Plac||4.0||8/22 pT181 attenuator||5.0||4.5
	|-		|-
-	|Ligation High buffer||5.0	+	|experiment||8/22 J23100||4.0||8/22 pT181 attenuator||5.0||4.5
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
-	|-	+
-	|8/21 DT||5.0	+
-	|-	+
-	|8/22 pT181 antisense||3.0	+
-	|-	+
-	|Ligation High buffer||4.0	+
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
-	|-	+
-	|8/21 DT||5.0	+
-	|-	+
-	|8/22 Spinach||3.0	+
-	|-	+
-	|Ligation High buffer||4.0	+
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
-	|-	+
-	|8/22 Plac||4.0	+
-	|-	+
-	|8/22 pT181 attenuator||5.0	+
-	|-	+
-	|Ligation High buffer||4.5	+
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
-	|-	+
-	|8/22 Pcon||5.0	+
-	|-	+
-	|8/22 pT181 attenuator||5.0	+
-	|-	+
-	|Ligation High buffer||5.0	+
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
-	|-	+
-	|8/22 Plac||4.0	+
-	|-	+
-	|8/22 pT181 antisense||8.0	+
-	|-	+
-	|Ligation High buffer||6.0	+
-	|}	+
-	{| class="wikitable"	+
-	!Name||-?-	+
	|-		|-
-	|8/22 Pcon||4.0	+	|experiment||8/22 Plac||4.0||8/22 pT181 antisense||8.0||6.0
	|-		|-
-	|8/22 pT181 antisense||8.0	+	|experiment||8/22 Plac||4.0||8/22 pT181 antisense||8.0||6.0
	|-		|-
-	|Ligation High buffer||6.0	+	|experiment||8/22 Pcon||4.0||8/22 pT181 antisense||8.0||6.0
	|}		|}
	</div>		</div>
-	<!-- ここまでをコピーしてね -->
	===Transformation===		===Transformation===

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Revision as of 14:25, 19 September 2013

Contents 1 Aug 26 1.1 Gel Extraction 1.2 Ligation 1.3 Transformation

Aug 26

Gel Extraction

Ligation

Transformation