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Revision as of 20:33, 12 June 2013

• Home
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• Results
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  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
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  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Phage Purification May - June Notebook: Experiments

Overview March-April May-June July-August September-October

6.10 Bacteria Cultures I) Purpose Grow bacteria for later use in preparation of phage. II) Expected Outcome Viable liquid cultures of both W3110 and BL21 III) Reagants Used W3110 and BL21 freezer stock LB IV) Actual Procedure Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37◦ C for 1 hour. Infect 4 mL of LB with 1 mL of liquid culture prepared in previous step. Let both liquid cultures sit overnight at 37◦ C. V) Results We were able to grow viable cultures of W3110 and BL21.