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Team:BYU Provo/Notebook/Cholera - Detection/Fallexp
From 2013.igem.org
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| - | <font size="3" font face="Calibri"> We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage. </font> | + | <font size="3" font face="Calibri"> We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage were also made. </font> |
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| - | <font size="3" font face="Calibri"> We did further work on purifying lambda. Also, we have been working on mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs. </font> | + | <font size="3" font face="Calibri"> We did further work on purifying lambda. Also, we have been working on purifying and mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs. </font> |
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Revision as of 03:00, 21 September 2013
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September 2 - September 15
We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage were also made.
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| September 16 - September 29
We did further work on purifying lambda. Also, we have been working on purifying and mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs.
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