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Team:BYU Provo/Notebook/Cholera - Detection/Summerexp
From 2013.igem.org
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Revision as of 04:43, 21 September 2013
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July 1 - July 14
We added Cro to our construct with the hope that overproduction of Cro will lead to the lysis of lambda.
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| July 15 - July 28
Add description!
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| July 29 - August 4
We have been having difficulties with lambda consistently going lytic. To correct this, we have plated 32 colonies from our original lambda containing strains to select for colonies that consistently go lytic when we provide stress. We found 3 strains that go lytic when we add H2O2. We will use those strains for our future tests.
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| August 5 - August 18
This week was spent trying to determine if lambda is stressed enough during electroporation to go lytic. If that is the case, it would explain why we never see plaques after doing an electroporation.
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| Aug 19 - Sept 1
This week we made a great discovery! If we plate our lambda containing e. coli next to cholera, lambda goes lytic!
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