Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

(Difference between revisions)
(→Ligation)
(→Ligation)
Line 6: Line 6:
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
|-
|-
-
|experiment||name||xx µg/mL||name||xx µg/mL||xx µg/mL
+
|experiment||Pconst||13.2||spiinach-DT||2.1L||3.5
|-
|-
-
|experiment||8/30 pSB1C3 (EcoRI & SpeI)||6.6||8/30 tRNA-Spinach (EcoRI & SpeI)||12.5||3.5
+
|experiment||Plac||15.3||spiinach-DT||2.1||3.5
|-
|-
-
|experiment||8/30 pSB1C3 (EcoRI & SpeI)||6.6||8/30 pT181 antisense (EcoRI & SpeI)||12.5||3.5
+
|experiment||Pconst||13.2||pT181 antisense (XbaI & PstI)||6.6||3.5
|-
|-
-
|experiment||8/30 pSB1C3 (XbaI & Pst)||12.5||8/30 pT181 attenuator (XbaI & PstI)||25||3.5
+
|experiment||Plac||15.3||pT181 antisense (XbaI & PstI)||6.6||3.5
|-
|-
-
|experiment||8/30 pSB1C3 (XbaI & Pst)||12.5||8/30 pT181 antisense (XbaI & PstI)||25||3.5
+
|experiment||Ptet||30.1||pT181 antisense (XbaI & PstI)||6.6||3.5
|-
|-
-
|experiment||8/20 DT (EcoRI & XbaI)||6.6||8/30 pT181 attenuator(2) (EcoRI & SpeI)||12.5||3.5
+
|experiment||Pconst||13.2||pT181 attenuator||11.7||3.5
|-
|-
-
|experiment||8/20 DT (EcoRI & XbaI)||6.6||8/30 pT181 antisense (EcoRI & SpeI)||12.5||3.5
+
|experiment||Plac||15.3||pT181 attenuator||11.7||3.5
|-
|-
-
|experiment||8/27 DT (EcoRI & XbaI)||3.0||8/30 tRNA-Spinach (EcoRI & SpeI)||12.5||3.5
+
|experiment||Ptet||30.1||RBS-lacZα-DT||3.0||3.5
|-
|-
-
|experiment||8/20 DT (EcoRI & XbaI)||6.6||8/30 RBS-lysis2 (EcoRI & SpeI)||12.5||3.5
 
-
|-
 
-
|experiment||8/22 J23100 (SpeI & PstI)||--||RBS-tetR-DT (XbaI & PstI)||--||3.5
 
-
|-
 
-
|experiment||8/22 Plac (SpeI & PstI)||8.0||8/18 RBS-lacZα-DT (XbaI & PstI)||10||3.5
 
-
|NC||name||xx µg/mL||name||xx µg/mL||xx µg/mL
 
|}
|}
 +
incubate 16°C 1hour
</div>
</div>

Revision as of 10:12, 23 September 2013

Aug 10

Ligation

Kojima

stateVectorInserterLigation High ver.2
experimentPconst13.2spiinach-DT2.1L3.5
experimentPlac15.3spiinach-DT2.13.5
experimentPconst13.2pT181 antisense (XbaI & PstI)6.63.5
experimentPlac15.3pT181 antisense (XbaI & PstI)6.63.5
experimentPtet30.1pT181 antisense (XbaI & PstI)6.63.5
experimentPconst13.2pT181 attenuator11.73.5
experimentPlac15.3pT181 attenuator11.73.5
experimentPtet30.1RBS-lacZα-DT3.03.5

incubate 16°C 1hour

Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Aug_10"