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Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
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!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
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| - | |experiment||Pconst||13.2||spiinach-DT||2. | + | |experiment||Pconst||13.2||spiinach-DT||2.1||3.5 |
|- | |- | ||
|experiment||Plac||15.3||spiinach-DT||2.1||3.5 | |experiment||Plac||15.3||spiinach-DT||2.1||3.5 | ||
Revision as of 21:30, 23 September 2013
Aug 10
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | Pconst | 13.2 | spiinach-DT | 2.1 | 3.5 |
| experiment | Plac | 15.3 | spiinach-DT | 2.1 | 3.5 |
| experiment | Pconst | 13.2 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
| experiment | Plac | 15.3 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
| experiment | Ptet | 30.1 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
| experiment | Pconst | 13.2 | pT181 attenuator | 11.7 | 3.5 |
| experiment | Plac | 15.3 | pT181 attenuator | 11.7 | 3.5 |
| experiment | Ptet | 30.1 | RBS-lacZα-DT | 3.0 | 3.5 |
incubate 16°C 1hour
