Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.26 Phage Titer
From 2013.igem.org
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : | + | : E Coli B freezer stock |
: LB | : LB | ||
: 1.5x top agar | : 1.5x top agar | ||
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'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
- | : Infect 500 µL of LB with 1 loop-full of | + | : Infect 500 µL of LB with 1 loop-full of E. Coli B freezer stock and put on shaker at 37<sup>◦</sup> C for 5 hours. |
: Plate w3110 on an LB plate with 4.5 mL top agar. | : Plate w3110 on an LB plate with 4.5 mL top agar. | ||
: Dilute T4 using a dilution series. | : Dilute T4 using a dilution series. |
Revision as of 21:13, 23 September 2013
Phage Purification July - August Notebook: Experiments
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7.26 Titer
I) Purpose
II) Expected Outcome
III) Reagants Used
V) Results
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