Template:Kyoto/Notebook/Aug 11

From 2013.igem.org

(Difference between revisions)
(→Miniprep)
(→Colony PCR)
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!Sample||base pair
!Sample||base pair
|-
|-
-
|(｀・ω・´)||
+
|BBa-B0034-(2)||-
 +
|-
 +
|BBa-B0034-(1)||-
 +
|-
 +
|BBa-R0011-(2)||-
 +
|-
 +
|BBa-R0011-(1)||-
 +
|-
 +
|negative control||-
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94°C||94°C||55°C||68°C||--
 +
|-
 +
|5min||30s||30s||20s||30
 +
|}
 +
</div>
 +
 
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|BBa-D0412-(1)||
 +
|-
 +
|BBa-D0412-(2)||
 +
|-
 +
|negative control||
|}
|}
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
+
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
|-
|-
-
|（◎Д◎）　||　||　||　||
+
|5min||30s||30s||1min30s||30
|}
|}
</div>
</div>
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<!-- ここまでをコピーしてね -->
 

Revision as of 06:48, 24 September 2013

Aug 11

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
8/9 BBa-J44000-(2)100
8/9 BBa-J23106154
8/9 BBa-J23100144
8/10 T7-His-FT92

Colony PCR

No name

Samplebase pair
BBa-B0034-(2)-
BBa-B0034-(1)-
BBa-R0011-(2)-
BBa-R0011-(1)-
negative control-
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s20s30
Samplebase pair
BBa-D0412-(1)
BBa-D0412-(2)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30s30

</div>

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