Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

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(→Waking Up Parts)
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===LB Medium Plate===
===LB Medium Plate===
 +
<div class="experiment">
 +
<span class="author">Nakamoto & Tatsui</span>
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{| class="wikitable"
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!volume||200ml
 +
|-
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|Bacto T2ypton || 2g
 +
|-
 +
|Bacto yeast extract ||1g
 +
|-
 +
|NaCl||1g
 +
|-
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|0.4&micro; NaOHaq||50&micro;L
 +
|-
 +
|Agar Pouder||2g　
 +
|}
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</div>
 +
===Transformation===
===Transformation===
===Gel Making===
===Gel Making===

Revision as of 06:42, 24 September 2013

Contents

Aug 10

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4µ NaOHaq50µL
Agar Pouder2g　

Transformation

Gel Making

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