Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

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===Transformation===
===Transformation===
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<div class="experiment">
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<span class="author">Hirano</span>
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{| class="wikitable"
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!Name||Sample||Competent Cells||Total||Plate
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|-
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|tetR||3&micro;L||19&micro;L||22&micro;L||Kan
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|-
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|RBS||3&micro;L||19&micro;L||22&micro;L||Amp
 +
|-
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|lacZ&alpha;||3&micro;L||19&micro;L||22&micro;L||CP
 +
|-
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|lacI||3&micro;L||19&micro;L||22&micro;L||CP
 +
|-
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|Plac||3&micro;L||19&micro;L||22&micro;L||CP
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|}
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</div>
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===Gel Making===
===Gel Making===

Revision as of 07:08, 24 September 2013

Contents

Aug 10

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g　
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP

Gel Making

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