Team:Hong Kong HKU/project/at a glance

From 2013.igem.org

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<b>Bacterial Microcompartments (BMCs)</b> are closed polyhedral macromolecular complexes with a diameter of 100-150 nm, enclosing enzymes and cofactors for various metabolism reactions. One of the example of such endogenous capsid micro-reactors is the <b><u>E</u>thanolamine <u>ut</u>ilization (Eut)</b> BMC from <i>Salmonella enterica</i> spp.
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Project AT a glance
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In <i>Salmonella enterica</i> spp., 5 genes (Eut S, M, N, L, K) encodes thousands of copies of shell proteins to form a heterogenous MCP shell. Such empty, recombinant Eut BMCs has been successfully cloned and expressed in <i>Escherichia coli</i>. In addition, a localization signal has been identified which can be fused into the N-terminus of unnaturally-encapsulated enzymes or proteins to facilitate their localization into the microcompartment
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Inspired by these studies, we speculated that this Eut BMC can become a versatile tool if we can:
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Phosphate pollution in waterways and water treatment plants is a major problem. Removal of phosphate from wastewater is required to treat phosphate-containing discharge to reduce eutrophication, algal blooms and “dead zones” in lakes, rivers and coastal marine ecosystems. The aim of this project was to remove or reduce the levels of inorganic phosphate from a system or environment by employing engineered bacteria E. capsi, capable of accumulating phosphate in the form of polyphosphate. Our strategy is to express polyphosphate kinase together with the ethanolamine utilization (eut) bacterial microcompartment from Salmonella entericato provide an environment for polyphosphate synthesis. Furthermore, the project provides a novel way to recover accumulated polyphosphate, an energy rich macromolecule with many industrial uses. This paves a way towards living system-based phosphate pollution treatment to tackle critical environmental challenges.
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<br>(a) modify the exterior surface to confer various novel physical, chemical, and biochemical properties to the whole capsule;
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<br>(b) localize special enzyme into the BMC for specfic functionalization;
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<br>(c) store or recycle useful and harmful molecules into the BMC
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<!---someone fix the alignment please, also note that the bolding and codings are not Wiki-styled, there must be some fundamental coding problems occuring here--->
<img src="https://static.igem.org/mediawiki/2013/8/87/BMC_with_His_tag_Fig.1_00000.jpg" width="240" height="216">
<img src="https://static.igem.org/mediawiki/2013/8/87/BMC_with_His_tag_Fig.1_00000.jpg" width="240" height="216">
<img src="https://static.igem.org/mediawiki/2013/5/52/PPK1_in_action_design_Fig.2_00000.jpg" width="240" height="216">
<img src="https://static.igem.org/mediawiki/2013/5/52/PPK1_in_action_design_Fig.2_00000.jpg" width="240" height="216">
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As a proof of concept, we displayed His tag on the surface of the Eut BMCs (Fig. 1), and at the same time, we fused the localization signal to polyphosphate kinase 1 from <i>Tannerella forsythia</i> (Fig. 2). After cloning both of the above constructs into <i>Escherichia coli</i>, we hope to demonstrate the one application of such integration by recycling phosphates from the polluted waters aided by the activity of polyphosphate kinase and encapsulating characteristics of Eut BMC.
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Introduction
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Bacterial MCPs are closed polyhedral shells 100-150nm diameter made of thin protein sheets, enclosing enzymes and cofactors for carbon fixation or various forms of fermentative metabolism. Recombinant Salmonella enterica ethanolamine utilization (Eut) bacterial MCP can be expressed heterologously in E.coli, both with and without the associated interior enzymes. A clonable localization N terminal signal enabling enzyme targeting to the MCP interior has been identified.  
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Revision as of 06:19, 25 September 2013


E. Capsi

At a Glance M1: Phosphate Removal M2: Polyphoshate Kinase M3: Micro-compartment Modelling References
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Project AT a glance

Phosphate pollution in waterways and water treatment plants is a major problem. Removal of phosphate from wastewater is required to treat phosphate-containing discharge to reduce eutrophication, algal blooms and “dead zones” in lakes, rivers and coastal marine ecosystems. The aim of this project was to remove or reduce the levels of inorganic phosphate from a system or environment by employing engineered bacteria E. capsi, capable of accumulating phosphate in the form of polyphosphate. Our strategy is to express polyphosphate kinase together with the ethanolamine utilization (eut) bacterial microcompartment from Salmonella entericato provide an environment for polyphosphate synthesis. Furthermore, the project provides a novel way to recover accumulated polyphosphate, an energy rich macromolecule with many industrial uses. This paves a way towards living system-based phosphate pollution treatment to tackle critical environmental challenges.

Introduction

Bacterial MCPs are closed polyhedral shells 100-150nm diameter made of thin protein sheets, enclosing enzymes and cofactors for carbon fixation or various forms of fermentative metabolism. Recombinant Salmonella enterica ethanolamine utilization (Eut) bacterial MCP can be expressed heterologously in E.coli, both with and without the associated interior enzymes. A clonable localization N terminal signal enabling enzyme targeting to the MCP interior has been identified.

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