Template:Kyoto/Notebook/Aug 10

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==Aug 10==
==Aug 10==
===Colony PCR===
===Colony PCR===
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<div class="experiment">
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<span class="author">Nakamoto</span>
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{| class="wikitable"
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!Sample
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|-
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|BBa_J23106
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|-
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|BBa_J23100
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|-
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|BBa_J44000
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|}
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{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
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|-
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|94 &deg;C||94 &deg;C||55 &deg;C||68 &deg;C||--
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|-
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|5 min||30 sec||30 sec||30 sec||30 cycles
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|}
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</div>
 +
===Electrophoresis===
===Electrophoresis===
===Liquid Culture===
===Liquid Culture===

Revision as of 04:23, 25 September 2013

Contents

Aug 10

Colony PCR

Nakamoto

Sample
BBa_J23106
BBa_J23100
BBa_J44000
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec30 sec30 cycles

Electrophoresis

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g　
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP

Gel Making

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