Team:UNITN-Trento/Notebook/Labposts/08/43

From 2013.igem.org

(Difference between revisions)

Revision as of 07:59, 25 September 2013

{ "date" : "2013-08-31", "author" : "fabio", "title" : " the blue circuit doesn’t always work!! ", "content" : " I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination. ", "tags" : "blue_light-EFE" }

@@ Line 2: / Line 2: @@
 	"date" : "2013-08-31",
 	"author" : "fabio",
-	"title" : " <html> EFE produced upon blue light illumination!!that’s totally awesome!!</html> ",
+	"title" : " <html> the blue circuit doesn’t always work!!</html> ",
-	"content" : " <html> in these last days we got our two final complete circuits!! Both my cloning and pedro’s succeeded so now we have a circuit that produces amilCP+EFE with blue light, and a circuit that produces amilGFP+EFE in the dark. We took some gas cromatografic measurements after an evaluated induction time with our LED to see if the devices actually produces ethylene. We could only see a really huge peak) ethylene in the sample under blue light with the final circuit with inverter. So the circuit with inverter seems to work, at least usually. We could not observe ethylene with the circuit that I cloned though.</html> ",
+	"content" : " <html> I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination.</html> ",
-	"tags" : "blue_light"
+	"tags" : "blue_light-EFE"
 }