Team:UNITN-Trento/Notebook/Labposts/08/43

From 2013.igem.org

(Difference between revisions)

Latest revision as of 09:15, 3 October 2013

{ "date" : "2013-08-15", "author" : "Michele-Viola", "title" : "Ferragosto in the lab... Impossibile!!", "content" : "Today we continued to work on Bacillus. The plates that we did yesterday were not so good so we decided to wait for another day to obtain a better growth. However we did a stock of competent Bacillus by starting from inocula did yesterday. These inocula were diluited in new and fresh transforamtion medium and then, when they reached OD about 0.9, glycerol was added and aliquote of 1 ml were done. So if everything will go as expected, finally we have obtained a stock of Mr Bacillus already competent. ", "tags" : "Bacillus" }

@@ Line 1: / Line 1: @@
 {
-"date":"2013-08-31",
+	"date" : "2013-08-15",
-"author":"fabio",
+	"author" : "Michele-Viola",
-"title":" <html> the blue circuit doesn’t always work!!</html> ",
+	"title" : "Ferragosto in the lab... Impossibile!!",
-"content":" <html> I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination.</html> ",
+	"content" : "<html>Today we continued to work on Bacillus. The plates that we did yesterday were not so good so we decided to wait for another day to obtain a better growth. However we did a stock of competent Bacillus by starting from inocula did yesterday. These inocula were diluited in new and fresh transforamtion medium and then, when they reached OD about 0.9, glycerol was added and aliquote of 1 ml were done. So if everything will go as expected, finally we have obtained a stock of Mr Bacillus already competent.   </html>",
-"tags":"blue_light-EFE"
+	"tags" : "Bacillus"
 }