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Latest revision as of 21:11, 19 June 2013

• Home
• Team
  • Overview
  • Official Team Profile
  • Team Video
  • Undergraduates
  • Mentors
• Project
  • Overview
  • Background
  • Experimental Design
• Notebook
  • Overview
  • Phage Team
  • Cholera Team
• Results
  • Overview
  • Judging Criteria
  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
  • Overview
  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Phage Purification May - June Notebook: Experiments

Overview March-April May-June July-August September-October

6.17 Phage Preparation I) Purpose Grow phage to practice purification procedures. II) Expected Outcome Viable stocks of both T4 and T7. III) Reagants Used W3110 and BL21 freezer stocks LB T4 and T7 phage stocks IV) Actual Procedure Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37◦ C overnight. Infect W3110 with 100 µL T4 and BL21 with 100 µL T7. Let both cultures sit overnight at 37◦ C. V) Results We created phage cultures to practice the purification procedure.