Team:Groningen/Labwork/25 September 2013

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
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Two colonies are chosen and inoculated in LB medium with Cm to higher the amount of the Pdes-cheY construct.  
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Two colonies are chosen and inoculated in LB medium with Cm to higher the amount of the Pdes-cheY construct. Did a miniprep on the samples.  
<br>Inoculations of &Delta;Des&Delta;CheY and WT bacillus strains in minimal medium.  
<br>Inoculations of &Delta;Des&Delta;CheY and WT bacillus strains in minimal medium.  
<br>Did a gel purification of CheC up and CheC down. Did restriction analysis of CheC up, CheC down and Spec. Ligation of CheC. Transformation of CheC in the &Delta;Des&Delta;CheY strain.   
<br>Did a gel purification of CheC up and CheC down. Did restriction analysis of CheC up, CheC down and Spec. Ligation of CheC. Transformation of CheC in the &Delta;Des&Delta;CheY strain.   

Latest revision as of 15:41, 25 September 2013

Mirjam

Two colonies are chosen and inoculated in LB medium with Cm to higher the amount of the Pdes-cheY construct. Did a miniprep on the samples.
Inoculations of ΔDesΔCheY and WT bacillus strains in minimal medium.
Did a gel purification of CheC up and CheC down. Did restriction analysis of CheC up, CheC down and Spec. Ligation of CheC. Transformation of CheC in the ΔDesΔCheY strain.