Template:Kyoto/Notebook/Sep 25

From 2013.igem.org

(Difference between revisions)
(Ligation)
(Sep 25)
Line 1: Line 1:
==Sep 25==
==Sep 25==
 +
 +
===Colony PCR===
 +
<div class="experiment">
 +
<span class="author">Nakamoto</span>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)||859
 +
|-
 +
|9/23 Pcon-PT181 attenuator-RBS-GFP-DT-(5~8)|1527
 +
|-
 +
|9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)||601
 +
|}
 +
===Transformation===
===Transformation===
-
<!-- こっから -->
 
<div class="experiment">
<div class="experiment">
<span class="author">kojima and nakamoto</span>
<span class="author">kojima and nakamoto</span>

Revision as of 09:38, 26 September 2013

Contents

Sep 25

Colony PCR

Nakamoto

Samplebase pair
9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)859
1527
9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)601

Transformation

kojima and nakamoto

NameSampleCompetent CellsTotalPlate
Plac+tet aptamer12+Pcon tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181attenuator-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-Spinach-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181antisense+DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181attenuator+tet aptamer12-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon tetR-DT+Pcon-GFP-DT+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181attenuator-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Ptet-pT181antisense+Spinach-DT+Pcon-RBS-GFP-DT+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Pcon-pT181antisense+Spinach-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Ptet-pT181antisense+Spinach-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-

NameSampleCompetent CellsTotalPlate
aptamer12-DT(EcoRI+Xbal)+Pcon-pT181attenuator(EcoRI+Spel)2µL20µL22µL-
Ptet(Spel+Pstl)+RBS-GFP-DT(Xbal+Pstl))2µL20µL22µL-

Ligation

nakamoto

stateVectorInserterLigation High ver.2
experiment9/25 aptamer12-xx µLnamexx µLxx µL
NCnamexx µLnamexx µLxx µL

|experiment||name||xx µL||name||xx µL||xx µL |- |NC||name||xx µL||name||xx µL||xx µL