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Team:Chiba/Project/store
From 2013.igem.org
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| - | パーツの事書く<br> | + | パーツの事書く<br><br><br><br><br> |
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| - | 実験結果書いていく | + | 実験結果書いていく<br><br><br><br> |
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Revision as of 09:52, 26 September 2013
Storage
1.Introduction
Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.
These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.
2.1.Plasmid
パーツの事書く
Part link
3.Result & Discussion
Assay
実験結果書いていく
