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Team:Chiba/Project/store
From 2013.igem.org
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<h2 id="store" style="background-color:#ff9933"><center>Storage</center></h2> | <h2 id="store" style="background-color:#ff9933"><center>Storage</center></h2> | ||
| - | <h3>1.Introduction</h3> | + | <h3 style="background-color:#ffdead ">1.Introduction</h3> |
<p> Fe(Ⅱ) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>. | <p> Fe(Ⅱ) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>. | ||
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| - | <h3>2.1.Plasmid</h3> | + | <h3 style="background-color:#ffdead ">2.1.Plasmid</h3> |
<p> | <p> | ||
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| - | <h3>2.2.Expression check</h3> | + | <h3 style="background-color:#ffdead ">2.2.Expression check</h3> |
<p> | <p> | ||
SDS pageについて書く | SDS pageについて書く | ||
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| - | <h3>3.Result & Discussion</h3> | + | <h3 style="background-color:#ffdead ">3.Result & Discussion</h3> |
<br><br><br> | <br><br><br> | ||
| - | <h3>3.1.鉄耐性評価</h3> | + | <h3 style="background-color:#ffe4c4 ">3.1.鉄耐性評価</h3> |
<p> | <p> | ||
<a href="#">Assay</a>実験方法別ページ<br> | <a href="#">Assay</a>実験方法別ページ<br> | ||
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</p> | </p> | ||
| - | <h3>3.2.鉄取り込み量評価</h3> | + | <h3 style="background-color:#ffe4c4 ">3.2.鉄取り込み量評価</h3> |
<p> | <p> | ||
<a href="#">Assay</a>実験方法別ページ<br> | <a href="#">Assay</a>実験方法別ページ<br> | ||
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</p> | </p> | ||
| - | <h3>3.3.磁性評価</h3> | + | <h3 style="background-color:#ffe4c4 ">3.3.磁性評価</h3> |
<p> | <p> | ||
<a href="#">Assay</a>実験方法別ページ<br> | <a href="#">Assay</a>実験方法別ページ<br> | ||
Revision as of 10:38, 26 September 2013
Storage
1.Introduction
Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.
These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.
2.1.Plasmid
パーツの事書く
Part link
2.2.Expression check
SDS pageについて書く
3.Result & Discussion
3.1.鉄耐性評価
Assay実験方法別ページ
実験結果書いてく
3.2.鉄取り込み量評価
Assay実験方法別ページ
実験結果書いてく
3.3.磁性評価
Assay実験方法別ページ
