Team:Tsinghua/Main-Page

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<h2>The Issue</h2>
<h2>The Issue</h2>
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Life-threatening pathogenic infections cause thousands of deaths every week all around the world. An effective and efficient identification of the infectious pathogens would facilitate the clinical treatment of these diseases. Nevertheless, current methods for identifying pathogens have many drawbacks including the long period required for test, the high cost resulted from multiple complex reactions and the lack of portable facilities. Clinical diagnosis of infectious pathogens would benefit significantly from specific and sensitive pathogen identification methods better than existing ones including pathogen culture, immunoassays and polymerase chain reaction (PCR) testing.  
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Life-threatening pathogenic infections cause thousands of deaths every week all around the world. The effective and efficient identification of the infectious pathogens would facilitate the clinical treatment of these diseases. Nevertheless, current methods for identifying pathogen have many drawbacks, such as long period for testing, multiple complex reactions caused high cost and lack of portable facilities. The clinical diagnosis of the infectious pathogens would benefit significantly from the specific and sensitive pathogen identification methods better than existing methods such as pathogen culture, immunoassays and polymerase chain reaction (PCR) testing.
</p>
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<h3>Opportunities for synthetic biology in mobile health</h3>
<h3>Opportunities for synthetic biology in mobile health</h3>
<p>
<p>
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Inspired by quorum sensing, a classical iGEM system under reconstruction and utilization, we designed a novel pathogen detection and identification system. The detecting system is made in the form of test paper, which can be carried by hand and used conveniently. More importantly, it’s much safer than the traditional iGEM detectors, as the system we use is S. cerevisiae (budding yeast). To accomplish the detection, people who suspect themselves to be infected only need to add a few drops of water as well as their pathogen-rich biological samples like snot or tears onto the test paper. Different color change of the test paper indicates different pathogen-specific infection. As some infections are caused by multiple pathogens, we constructed a switch-box composed of different sensors and different reporters in order to detect multiple pathogens at the same time. This system increases the speed and portability of pathogen identification method, decreases the cost for producing practical facilities, and enables specific identification of distinct infectious pathogens. The challenge is addressed by implementing the genetically modified S. cerevisiae (budding yeast) for testing signal molecules from pathogens. The system is supposed to be effective, reliable, safe and cost-effective.
+
By the inspiration of mechanisms of quorum sensing, the classical iGEM system under reconstruction and utilization, We designed a novel pathogen detection and identification system, it is quiet convenient as the sensing system is made in the form of test paper, which can be carried at hand. More importantly, it is much more safer than the traditional iGEM detectors, as the system we use is S. cerevisiae (budding yeast) . To accomplish the detection, the suspected patients only need to add a little bit water as well as the pathogen-riched biological sample like snot and tears. The color change of the test paper indicate the infection by specific pathogen. Also, as some infections are caused by multiple pathogens, in order to detect the different pathogens at the same time, we construct a switch-box, which is composed of different sensors and different reporters. This system could increase the speed and portability of pathogen identification, decrease the cost for producing practical facilities, and enable specific identification of distinct infectious pathogens. The challenge is addressed by implementing the genetically modified S. cerevisiae (budding yeast) for testing signal molecules from pathogens, which is supposed to be effective, reliable, safe and cost-effective.
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<h2>Idea of the project</h2>
<h2>Idea of the project</h2>
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The project introduces the use of genetically engineered cells in a pathogen identification system. By virtue of the switchable haploid/diploid form of yeast, two kinds of haploid budding yeast have been constructed, one for sensing the signal molecules from pathogens and the other reporting the identification of pathogens, referred as sensing module and reporting module, respectively. The sensing module and reporting module are combined together by mating between the two differently engineered yeasts, and the signals from pathogens are sensed and reported by change in the color of the yeast colony. N-Acyl Homoserine Lactone (AHL) is the signal to be tested by the sensing module. It is the molecule for quorum sensing in Gram-negative bacteria with specificity for each different species of pathogens. Tetracycline-controlled transcriptional activation system (Tet-off system) is introduced to transmit signals from the sensing module to the reporting module. Considering the specificity and sensitivity of this pathogen identification sensor, this technology could be useful for clinical test, medical diagnostics, water quality monitoring, and other potential applications.
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The project introduces the use of genetically engineered cells in a pathogen identification system. By virtue of the switchable haploid/diploid form of yeast , we aim to construct two kinds of haploid budding yeast, one is  to sense the signal molecules from pathogens , the other is to report the identification of pathogens, referred as sensing module and reporting module, respectively. By mating the sensing module and reporting module, two differently engineered yeasts, the signals from pathogens are sensed and reported by change in the color of the yeast colony. The N-Acyl Homoserine Lactone (AHL) is the signal for testing by the sensing module, which is the molecule for quorum sensing in Gram-negative bacteria with specificity for each different species of pathogens. Tetracycline-controlled transcriptional activation system (Tet-off system) is introduced to transmit signals from sensing module to reporting module. Considering the specificity and sensitivity of this pathogen identification sensor, this technology could prove useful for clinical test, medical diagnostics, water quality monitoring, and other potential applications.
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We accomplished the communication between eukaryotic cells and prokaryotic cells for the first time via introducing the genetically modified quorum sensing system from Gram-negative bacteria (LuxR-AHL controlled transcriptional activation system) to S. cerevisiae (budding yeast) by designing eukaryotic-prokaryotic mosaic transcriptional activator and promoter, referred as LuxR-tVP16 and Plux-mCyc respectively, which provided more possibilities for future iGEM projects.
+
We accomplish the communication between eukaryotic cells and parkaryotic cells in the first time via introduced the genetically modified quorum sensing system from Gram-negative bacteria (LuxR-AHL controlled transcriptional activation system) to S. cerevisiae (budding yeast) by designing eukaryotic-parkaryotic mosaic transcriptional activator and promoter, referred as LuxR-tVP16 and Plux-mCyc respectively. Which expand the possibility of iGEM projects in the future.
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<li>We designed and completed a switching system by mating yeasts containing different reporting modules with those containing a sensing module.
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We designed and completed the switching system by mating different reporting modules with the sensing module.
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We constructed a mathematical model to demonstrate the sensitivity and robustness of this system combining modified quorum sensing and tetracycline controlled transcriptional activation systems.  
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We constructed mathematical modeling to demonstrate the sensitivity and robustness of this system with combination of modified quorum sensing and tetracycline controlled transcriptional activation systems.
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We introduced several safety mechanisms to this engineered biological testing machine.
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We deposited several novel BioBricks to the Registry.
We deposited several novel BioBricks to the Registry.
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We improved several existing BioBricks.
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We improved the existing BioBricks.
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We introduced the project and synthetic biology to the society by activities including making videos.
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We introduce the project and synthetic biology to society by making videos and other activities.
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Revision as of 18:45, 26 September 2013

Main Page

Pathogen detection is one major topic related to the access to health care, the failingof which leads to serious consequences. Pseudomonasaeruginosa and Staphylococcus aureusare two problematic pathogenic Gram-negative bacteria causing various diseases.Fast and sensitive detection of them is required for rapidly administered andappropriate antibiotic treatment in serious medical conditions.

By the inspiration of quorum sensing system, we designed a novel pathogen detectionsystem.  Considering the specificityand sensitivity of quorum sensing, this technology could prove useful forclinical test, medical diagnostics, and other potential applications.

  • The Issue
  • Idea of the project
  • Achievement

The Issue

Life-threatening pathogenic infections cause thousands of deaths every week all around the world. The effective and efficient identification of the infectious pathogens would facilitate the clinical treatment of these diseases. Nevertheless, current methods for identifying pathogen have many drawbacks, such as long period for testing, multiple complex reactions caused high cost and lack of portable facilities. The clinical diagnosis of the infectious pathogens would benefit significantly from the specific and sensitive pathogen identification methods better than existing methods such as pathogen culture, immunoassays and polymerase chain reaction (PCR) testing.

Opportunities for synthetic biology in mobile health

By the inspiration of mechanisms of quorum sensing, the classical iGEM system under reconstruction and utilization, We designed a novel pathogen detection and identification system, it is quiet convenient as the sensing system is made in the form of test paper, which can be carried at hand. More importantly, it is much more safer than the traditional iGEM detectors, as the system we use is S. cerevisiae (budding yeast) . To accomplish the detection, the suspected patients only need to add a little bit water as well as the pathogen-riched biological sample like snot and tears. The color change of the test paper indicate the infection by specific pathogen. Also, as some infections are caused by multiple pathogens, in order to detect the different pathogens at the same time, we construct a switch-box, which is composed of different sensors and different reporters. This system could increase the speed and portability of pathogen identification, decrease the cost for producing practical facilities, and enable specific identification of distinct infectious pathogens. The challenge is addressed by implementing the genetically modified S. cerevisiae (budding yeast) for testing signal molecules from pathogens, which is supposed to be effective, reliable, safe and cost-effective.

Idea of the project

The project introduces the use of genetically engineered cells in a pathogen identification system. By virtue of the switchable haploid/diploid form of yeast , we aim to construct two kinds of haploid budding yeast, one is to sense the signal molecules from pathogens , the other is to report the identification of pathogens, referred as sensing module and reporting module, respectively. By mating the sensing module and reporting module, two differently engineered yeasts, the signals from pathogens are sensed and reported by change in the color of the yeast colony. The N-Acyl Homoserine Lactone (AHL) is the signal for testing by the sensing module, which is the molecule for quorum sensing in Gram-negative bacteria with specificity for each different species of pathogens. Tetracycline-controlled transcriptional activation system (Tet-off system) is introduced to transmit signals from sensing module to reporting module. Considering the specificity and sensitivity of this pathogen identification sensor, this technology could prove useful for clinical test, medical diagnostics, water quality monitoring, and other potential applications.

Achievement

  • We accomplish the communication between eukaryotic cells and parkaryotic cells in the first time via introduced the genetically modified quorum sensing system from Gram-negative bacteria (LuxR-AHL controlled transcriptional activation system) to S. cerevisiae (budding yeast) by designing eukaryotic-parkaryotic mosaic transcriptional activator and promoter, referred as LuxR-tVP16 and Plux-mCyc respectively. Which expand the possibility of iGEM projects in the future.
  • We designed and completed the switching system by mating different reporting modules with the sensing module.
  • We constructed mathematical modeling to demonstrate the sensitivity and robustness of this system with combination of modified quorum sensing and tetracycline controlled transcriptional activation systems.
  • We introduced several safety mechanisms to this engineered biological testing machine.
  • We deposited several novel BioBricks to the Registry.
  • We improved the existing BioBricks.
  • We introduce the project and synthetic biology to society by making videos and other activities.

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