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Team:Freiburg/Project/modeling
From 2013.igem.org
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TestVar = TestVar.replace(/</g,"\n <"); | TestVar = TestVar.replace(/</g,"\n <"); | ||
| - | var w = window.open("blank.html", "blank", " | + | var w = window.open("blank.html", "blank", "toolbar=yes, location=yes, directories=no, status=no, menubar=yes, scrollbars=yes, resizable=no, copyhistory=yes, width=1000, height=700"); |
w.document.writeln("<h2> The inserted sequence is: </h2>"); | w.document.writeln("<h2> The inserted sequence is: </h2>"); | ||
w.document.writeln(TestVar); | w.document.writeln(TestVar); | ||
Revision as of 22:44, 26 September 2013
crRNA-Design
This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to the BbsI-overhangs by pX334a.
For repression of gene transcription by targeting the coding sequence
it´s crucial to target the non template (= coding) DNA strand.
Therefore the oligos must be designed as
follows:
- Search at your desired target sequence for a CCN (reverse complement of the PAM sequence) at the coding strand.
- Extract the following (3') 30 nucleotides.
- Extract the reverse complement.
- Add AAAC at the 5' end and GT at the 3' end. This will be your fist oligo.
- Take the sequence from step 2 and add TAAAAC at the 5' end. This will be your second oligo.
