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Team:Buenos Aires/ res rfpdeg
From 2013.igem.org
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''GFP production in relation with arsenite concentration'' | ''GFP production in relation with arsenite concentration'' | ||
| - | To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb.)Fluorescence was then measured with a fluorimeter at 395 nm excitation peak and | + | To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb.)Fluorescence was then measured with a fluorimeter at 395 nm excitation peak and 515 nm emission peak. All was normalized by the culture density measured by OD (600nm). |
'''Results ''' | '''Results ''' | ||
Revision as of 16:04, 27 September 2013
GFP under Arsenic Inducible Promoter
GFP production in relation with arsenite concentration
To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb.)Fluorescence was then measured with a fluorimeter at 395 nm excitation peak and 515 nm emission peak. All was normalized by the culture density measured by OD (600nm).
Results
As it can be seen in the graphics bellow the arsenite sensible promoter is correspondingly active in relation with the arsenite concentration.
