Team:KIT-Kyoto/Notebook/growcurves

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Growth curves

August 20^th,21^st

We transformed four kinds of plasmid DNA into E.coli cells.

These plasmids were obtained from iGEM DNA distributions kit (listed below).

We added 10ul of water into the samples and left them for 5 minutes before using the parts.

(1)2012KitPlate2,6O(pSB1A3)

(2)2012KitPlate2,8A(pSB1A2)

(5)2013KitPlate5,15J(pSB1A2)

(6)2013KitPlate5,24E(pSB1A2)

We obtained colonies of sample (1), (2), and (6), but did not "5".

We prepared sample (5) again and got it.

We cultured each transformant in 3 ml LB liquid medium containing ampicillin (37°C, 125rpm overnight).

August 23^rd~September 3^rd

We measured the turbidities of the transformants (OD₆₀₀).

We transferred the transformant prepared on 8/22 to 200 mL flasks.

We measured the turbidities every 30 minutes.

But we could not get positive data.

The data are indicated below.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

We measured the turbidities of the transformants (OD₆₀₀).

We transferred the transformant prepared on 8/25 to 200 mL flasks.

We measured the turbidities every 30 minutes.

But we could not get positive data.

The data are indicated below.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

We measured the turbidities of the transformants (OD₆₀₀).

We transferred the transformant prepared on 8/26 to 200 mL flasks.

We measured the turbidities every 30 minutes.

But we could not get positive data.

The data are indicated below.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

We measured the turbidities of the transformants (OD₆₀₀).

We transferred the transformant prepared on 9/2 to 200 mL flasks.

We measured the turbidities every 30 minutes.

But we could not get positive data.

The data are indicated below.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

September 4^th~7^th

We measured the turbidities of the transformants at hourly intervals and made the growth curves.

We transferred the transformant prepared on 9/3 to 200 mL flasks.

We measured the turbidities every 1 hour.

The measurements were carried out for 12 hours.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

37°C, 125rpm

Control:HST08

We measured the turbidities of the transformants at hourly intervals and made the growth curves.

We transferred the transformant prepared on 9/4 to 200 mL flasks.

We measured the turbidities every 1 hour.

The measurements were carried out for 12 hours.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

37°C, 125rpm

Control:HST08

We measured the turbidities of the transformants at hourly intervals and made the growth curves.

We transferred the transformant prepared on 9/5 to 200 mL flasks.

We measured the turbidities every 1 hour.

The measurements were carried out for 12 hours.

Liquid LB	50mL
Sample	20μL
Ampicillin	75μL

37°C, 125rpm

Control:HST08

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