Template:Freiburg2013 Template

From 2013.igem.org

(Difference between revisions)
m
Line 3: Line 3:
<style type="text/css">
<style type="text/css">
-
  .firstHeading {                                                    /* Gets rid of the default iGEM picture*/
+
.firstHeading {                                                    /* Gets rid of the default iGEM picture*/
     height:2px;
     height:2px;
     visibility:hidden;
     visibility:hidden;
-
}  
+
}  
#contentSub, #search-controls, .firstHeading, #catlinks, #p-logo, .editsection {
#contentSub, #search-controls, .firstHeading, #catlinks, #p-logo, .editsection {
     display:none;
     display:none;
-
}
+
}
            
            
#content {
#content {
-
background-color: transparent;
+
background-color: transparent;
     border-left-width:0px;
     border-left-width:0px;
     border-right-width:0px;
     border-right-width:0px;
-
     padding: 0px 5px 0px 5px;
+
     padding: 0px 5px 0px 5px;                                              
-
                                                 
+
}
-
}
+
-
#footer-box {
+
#footer-box {
-
+
background:none;
-
background:none;
+
border:none;
-
border:none;
+
}
-
}
+
#menubar {
#menubar {
display: table-cell;
display: table-cell;
background: transparent;
background: transparent;
-
margin-top:90px;
+
margin-top:90px;
-
        margin-left: 200px;
+
    margin-left: 200px;
-
        margin-right: 230px;
+
    margin-right: 230px;
-
}
+
}
-
 
+
#menubar * {
#menubar * {
-
color: transparent !important;
+
color: white !important;
-
background: transparent !important;
+
background: white !important;
-
}
+
}
#menubar:hover * {
#menubar:hover * {
-
color: #C0C0C0 !important;
+
color: white !important;
-
}
+
}
-
#p-logo { height 1px; overflow:hidden; display: none;
+
#p-logo {  
-
}
+
overflow:hidden;  
 +
display: none;
 +
}
-
#top-section  
+
#top-section {
-
{
+
heigth:0px;
-
heigth:0px;
+
width: 100%;
-
width: 100%;
+
margin:0px;
-
margin:0px;
+
padding: 0px;
-
  padding: 0px;
+
     background-repeat: no-repeat;
     background-repeat: no-repeat;
     border-width:0px;
     border-width:0px;
     border-top-width:0px;
     border-top-width:0px;
-
}
+
}
 +
 
#toc{
#toc{
-
visibility:hidden;
+
visibility:hidden;
-
display:none;
+
display:none;
-
}
+
}
 +
 
#siteSub {
#siteSub {
-
display:none;
+
display:none;
-
}
+
}
 +
#search-controls {                                                 
#search-controls {                                                 
-
display:none;
+
display:none;
-
margin-top:0px;
+
margin-top:0px;
-
}
+
}
 +
#contentSub {
#contentSub {
-
display:none;
+
display:none;
-
}
+
}
/*Now to set heading and body fonts*/
/*Now to set heading and body fonts*/
body {
body {
-
width:100%;
+
width:1000px;
-
    background: #FFFFFF ;                      
+
/* background: url("Hintergrund_linear.gif") center top;*/
-
padding: 0;
+
background-color: #2E2E2E;
-
   margin-top:-80px;
+
-
} 
+
    background-width: 100%;          
-
#navbar img {
+
   margin-top:0px; /*-80px*/
-
  visibility:hidden;
+
 
-
  position:absolute;
+
  float:center;
-
  width: 100px;
+
  postion: absolute;
-
   top:-80px;
+
 
-
}
+
}    
-
#bild span {visibility:visible;}
+
#menu {  
#menu {  
 +
width:100%;
 +
height: 3em;
 +
float: left;
 +
position: abolute;
 +
margin-top:150px;
 +
background-color: transparent;
-
background-image:url(DNA1.gif);
+
background-repeat: no-repeat;
-
background-repeat: no-repeat;
+
-
background-position: -170px 45px;
+
border-bottom: 8px #FFBF00 solid;
-
background-size: 1500px 100px;
+
-
width:82%;
+
}
-
+
-
height: 3.8em;
+
-
float: center;
+
-
padding-left: 100px;
+
-
padding-top: 60px;
+
-
 
+
-
+
-
}
+
#menu ul li {
#menu ul li {
-
 
+
background-color:transparent;
-
background-color:transparent;
+
  list-style: none;
-
  list-style: none;
+
  float: left;  
-
  float: left;  
+
 
 +
   
}
}
#menu ul li a {  
#menu ul li a {  
 +
background-color: #2E2E2E;
-
background-color:transparent;
 
-
  padding-left: 10px;
 
padding-bottom: 5px;
padding-bottom: 5px;
   padding-right: 20px;
   padding-right: 20px;
   padding-top: 5px;
   padding-top: 5px;
   display: block;
   display: block;
-
   color: black;  
+
 
 +
   color:white ;  
   text-decoration: none;  
   text-decoration: none;  
   font-weight:bold;
   font-weight:bold;
-
font-family: Gill Sans MT;
+
font-family: Arial;
-
font-size: 12pt;
+
font: 1.2em/1.5em;
-
font-color:black;
+
 +
/*text-shadow:#FFBF00 1px 2px;*/
 +
letter-spacing:0.1em;
}
}
-
+
#menu a:hover {
-
    #menu a:hover {
+
background: #2E2E2E;
-
    background: #C0C0C0;
+
color:#FFBF00;
-
 
+
}
-
    }
+
-
    #menu li {
+
#menu li {
     position: relative;
     position: relative;
     float: center;  
     float: center;  
     }  
     }  
-
  #navbar ul {  
+
#navbar ul {  
     position: absolute;  
     position: absolute;  
     z-index: 500;  
     z-index: 500;  
     }
     }
-
    #navbar ul ul { position: absolute; top: 10px; left: 50%; }  
+
#navbar ul ul {  
 +
position: absolute;  
 +
top: 10px;  
 +
left: 50%;  
 +
}  
-
    #navbar ul,
+
#navbar ul,
-
    #navbar li:hover ul ul,
+
#navbar li:hover ul ul,
-
    #navbar ul li:hover ul ul
+
#navbar ul li:hover ul ul{
-
      {display: none;
+
display: none;
-
     
+
       }  
       }  
-
    #navbar  li:hover ul,
+
#navbar  li:hover ul,
-
    #navbar ul li:hover ul,
+
#navbar ul li:hover ul,
-
    #navbar ul ul li:hover ul,
+
#navbar ul ul li:hover ul,
-
#navbar li:hover img
+
#navbar li:hover img {
-
      {display: block;
+
display: block;
-
visibility:visible;
+
visibility:visible;
-
margin: 0;
+
margin: 0;
-
  padding: 0; }  
+
padding: 0;  
 +
}  
 +
#logo_off{
 +
z-index:2;
 +
position:absolute;
 +
float: right;
 +
width:7%;
 +
margin-right:0px;
 +
margin-top:0px;
 +
margin-left:900px;
 +
}
-
#logo {
 
-
float:left;
 
-
width: 10%;
 
-
}
 
 +
#logo {
 +
z-index:1;
 +
position:absolute;
 +
}
 +
 +
#logo img{
 +
 +
float:left;
 +
width:1000px;
 +
 +
 +
}
-
#offwiki {
+
#left {
-
 
+
padding-left:20px;
-
z-index: 10;
+
padding-top: 50px;
-
width:10%;
+
width:50%;
-
float: right;
+
float:left;
-
+
position:relative;
-
}
+
color:white;
-
 
+
font-family: Arial;
-
#header {padding-top:0px;}
+
text-align:justify;
-
 
+
}
-
 
+
-
#Text{padding-top: 110px;}
+
 +
#right {
 +
padding-top: 100px;
 +
width:40%;
 +
float:right;
 +
position:relative;
 +
}
 +
 +
#h1 {
-
#Fusszeile {
+
font-color:white;
-
    clear: both;
+
}
-
    font-size: 0.9em;
+
-
    margin: 0; padding: 0.1em;
+
-
    text-align: center;
+
-
   
+
-
  }
+
-
 
+
-
#right {float:right;
+
-
width:30%;}
+
-
+
-
#left {float:left;
+
-
width:70%;}
+
    
    
</style>
</style>
 +
<script>
<script>
sfHover = function()  
sfHover = function()  
Line 221: Line 239:
-
<body>    
+
<body>  
 +
<div id="header">
<div id="header">
-
<div id="logo"><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/></div>
+
<div id="logo" style="z-index: 8;"><img src="Logo.png"/></div>
-
+
     <div id="logo_off" style="z-index: 10;"><a href="https://2013.igem.org/Main_Page"><img src="IGEM_Logo.gif" width=100%/></a></div>
-
     <div id="offwiki"><a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%"/></a></div>
+
    
    
     </div>
     </div>
Line 231: Line 249:
   <div id="menu">
   <div id="menu">
       <ul id="navbar">
       <ul id="navbar">
-
         <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg">Home</a>
+
         <li><a href="https://2013.igem.org/Team:Freiburg">Home</a>
         </li>
         </li>
-
         <li><a href="https://2013.igem.org/Team:Freiburg/Team">Team</a><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/>
+
         <li><a href="https://2013.igem.org/Team:Freiburg/Team">Team</a>
         <ul>
         <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Team2">Members</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Team2">Members</a></li>
Line 240: Line 258:
             </ul>
             </ul>
         </li>
         </li>
-
         <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg/Project">Project</a>  
+
         <li><a href="https://2013.igem.org/Team:Freiburg/Project">Project</a>  
         <ul>
         <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/1">1</a>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/1">1</a>
-
              <ul>
+
              <!--  <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/1/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/1/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/1/Results">Results</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/1/Results">Results</a></li>
-
               </ul>
+
               </ul> -->
               </li>
               </li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/2">2</a>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/2">2</a>
-
              <ul>
+
            <!--  <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/2/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/2/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/2/Results">Results</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/2/Results">Results</a></li>
-
               </ul>
+
               </ul> -->
               </li>
               </li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/3">3</a>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/3">3</a>
-
              <ul>
+
          <!--    <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/3/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/3/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/3/Results">Results</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/3/Results">Results</a></li>
-
               </ul>
+
               </ul> -->
               </li>
               </li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/4">4</a>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/4">4</a>
-
              <ul>
+
            <!--  <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/4/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/4/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/4/Results">Results</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/4/Results">Results</a></li>
-
               </ul>
+
               </ul> -->
               </li>
               </li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/5">5</a>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/5">5</a>
-
              <ul>
+
          <!--    <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/5/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/5/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/5/Results">Results</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/5/Results">Results</a></li>
-
               </ul>
+
               </ul> -->
               </li>
               </li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/6">6</a>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/6">6</a>
-
              <ul>
+
              <!--  <ul>
-
              <li><a href="https://2013.igem.org/Team:Freiburg/Project/6/Labbook">Labbook</a></li>
+
              <li><a href="https://2013.igem.org/Team:Freiburg/Project/6/Labbook">Labbook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/6/Results">Results</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Project/6/Results">Results</a></li>
-
               </ul>
+
               </ul> -->
               </li>
               </li>
             </ul>       
             </ul>       
         </li>
         </li>
-
         <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg/HumanPractice">Human Practice</a>  
+
         <li><a href="https://2013.igem.org/Team:Freiburg/HumanPractice">Human Practice</a>  
         <ul>
         <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/HumanPractice/Facebook">Facebook</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/HumanPractice/Facebook">Facebook</a></li>
             </ul>       
             </ul>       
         </li>
         </li>
-
  <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg/Modeling">Modeling</a>
+
  <li><a href="https://2013.igem.org/Team:Freiburg/Modeling">Modeling</a>
  <ul>
  <ul>
               <li><a href="https://2013.igem.org/Team:Freiburg/Modeling/crRNADesign">crRNADesign</a></li>
               <li><a href="https://2013.igem.org/Team:Freiburg/Modeling/crRNADesign">crRNADesign</a></li>
             </ul>         
             </ul>         
         </li>
         </li>
-
         <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg/Notebook">Notebook</a>
+
         <li><a href="https://2013.igem.org/Team:Freiburg/Notebook">Notebook</a>
         </li>
         </li>
-
         <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg/Safety">Safety</a>  
+
         <li><a href="https://2013.igem.org/Team:Freiburg/Safety">Safety</a>  
         </li>
         </li>
-
         <li><img src="https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png" width="100%";/><a href="https://2013.igem.org/Team:Freiburg/Attributions">Attributions</a>
+
         <li><a href="https://2013.igem.org/Team:Freiburg/Attributions">Attributions</a>
         </li>
         </li>
       </ul>   
       </ul>   
Line 300: Line 318:
    
    
-
<div id="Text">
 
-
 
 +
<div id="left">
 +
<h1 id="h1">Project Description</h1>
 +
<p><font size="5">I</font>f one fact is true: Biology proved itself to be more complex than we could ever have imagined. This especially holds true for fields like cancer research and cell reprogramming.
 +
Up to several years ago the main approach for investigating gene signaling cascades was to focus on single genes and underestimating crosstalk in between pathways. Investigating on those crosstalks was a difficult, time consuming work, that is up to date a challenge for scientists all around the world. High throughput methods like whole transcriptome analysis gave rise to complete new approaches and ideas. One major flaw of these methods is, that they are only descriptive and do not allow to interfere with those systems.</p>
 +
 +
<p>Here, we demonstrate a system, based on a protein-RNA-DNA interaction that allows multiple gene regulation simultaneously with minimal effort. Cas9, a nuclease, derived from a bacterial immunity system, is able to bind to multiple RNA specified targets.
 +
The FreiGEM 2013 team aims to develop from this protein a new class of DNA binding proteins by mutating the nuclease and by fusing it to effector domains. The aim was not only to modify gene expression, but also to make this process inducible in a spatiotemporal manner. This gives rise to application in medical and fundamental research.</p>
 +
 +
<p>For a proof-of-principle work we demonstrate function in human cell lines and evaluated those results by theoretical modeling.
 +
Furthermore, we were able to manipulate endogenous targets in the well-studied model organisms Danio rerio and Arabidopsis thaliana, by targeting factors involved into stem cell reprogramming stem cells were induced and endogenous reporters, respectively.</p>
 +
 +
 +
 +
</div>
 +
 +
<div id="right">
 +
<img src="CRISPR.gif" width="400px">
</div>     
</div>     
Line 310: Line 343:
</body>
</body>
 +
 +
</html>

Revision as of 17:30, 3 August 2013

Project Description

If one fact is true: Biology proved itself to be more complex than we could ever have imagined. This especially holds true for fields like cancer research and cell reprogramming. Up to several years ago the main approach for investigating gene signaling cascades was to focus on single genes and underestimating crosstalk in between pathways. Investigating on those crosstalks was a difficult, time consuming work, that is up to date a challenge for scientists all around the world. High throughput methods like whole transcriptome analysis gave rise to complete new approaches and ideas. One major flaw of these methods is, that they are only descriptive and do not allow to interfere with those systems.

Here, we demonstrate a system, based on a protein-RNA-DNA interaction that allows multiple gene regulation simultaneously with minimal effort. Cas9, a nuclease, derived from a bacterial immunity system, is able to bind to multiple RNA specified targets. The FreiGEM 2013 team aims to develop from this protein a new class of DNA binding proteins by mutating the nuclease and by fusing it to effector domains. The aim was not only to modify gene expression, but also to make this process inducible in a spatiotemporal manner. This gives rise to application in medical and fundamental research.

For a proof-of-principle work we demonstrate function in human cell lines and evaluated those results by theoretical modeling. Furthermore, we were able to manipulate endogenous targets in the well-studied model organisms Danio rerio and Arabidopsis thaliana, by targeting factors involved into stem cell reprogramming stem cells were induced and endogenous reporters, respectively.

Retrieved from "http://2013.igem.org/Template:Freiburg2013_Template"